中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (28): 5216-5219.doi: 10.3969/j.issn.1673-8225.2010.28.020

• 组织构建实验造模 experimental modeling in tissue construction • 上一篇    下一篇

构建股骨头缺血坏死模型兔总胆固醇和三酰甘油的变化

张宇博，彭  昊，方洪松，李皓桓，汪  巍   

  1. 武汉大学人民医院骨Ⅱ科，湖北省武汉市  430060
  • 出版日期:2010-07-09 发布日期:2010-07-09
  • 作者简介:张宇博★，男，1984年生，湖北省武汉市人，汉族，武汉大学在读硕士，主要从事股骨头缺血性坏死方向的研究。 1049756476@qq.com

Changes of total cholesterol and triacylglycerol in rabbit models of avascular necrosis of the femoral head

Zhang Yu-bo, Peng Hao, Fang Hong-song, Li Hao-huan, Wang Wei   

  1. Second Department of Orthopedics, Renmin Hospital of Wuhan University, Wuhan  430060, Hubei Province, China
  • Online:2010-07-09 Published:2010-07-09
  • About author:Zhang Yu-bo★, Studying for master’s degree, Second Department of Orthopedics, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei Province, China 1049756476@qq.com

PDF

525

被引次数

5

摘要：

背景：引起股骨头坏死最常见的非创伤性致病因素是激素的大量使用，尽管激素与非创伤性股骨头坏死的关系密切，但其发病机制至今尚未阐明。
目的：通过建立股骨头缺血坏死动物模型并检测脂质代谢指标，以探讨脂质代谢与股骨头坏死的关系。
方法：20只健康日本大白兔随机分成2组。实验组通过联合应用马血清和甲泼尼龙建立股骨头坏死的动物模型。对照组腹腔注射生理盐水。于开始注射甲泼尼龙针后第2，4，6，8周检测血清中总胆固醇和三酰甘油；于第2，4，6周分别处死实验组及对照组各2只动物行苏木精-伊红染色组织学检查；于第8周，剩余动物行DR、CT检查后处死行组织学检查。
结果与结论：实验组DR检查显示双侧骨密度降低，骨骺线不清，骨质网状结构模糊；CT显示骨质网状结构密度降低，呈点状分布，骨皮质变薄，骨质疏松，骨骺线不清；苏木精-伊红染色显示骨小梁结构不清，排列紊乱，骨髓内脂肪细胞增多，骨细胞缺失，血栓形成，肥大细胞增生，而对照组的DR、CT和苏木精-伊红染色未见明显异常。实验组造模后总胆固醇、三酰甘油升高(P < 0.05)，且明显高于对照组(P < 0.01)。结果证实，联合应用马血清和激素可成功建立股骨头坏死动物模型。脂质代谢紊乱是激素性股骨头坏死重要发病机制之一。

关键词: 股骨头坏死, 脂质代谢, 总胆固醇, 三酰甘油, 骨组织工程

Abstract:

BACKGROUND: Slather of glucocorticoid (GC) is the most common non-traumatic cause for necrosis of the femoral head. Though there are close association between GC and necrosis of the femoral head, the underlying mechanisms remain unclear.
OBJECTIVE: To establish an experimental model of avascular necrosis of the femoral head (ANFH) caused by corticosteroid in adult rabbits and to observe the metabolism of fatty substance.
METHODS: Twenty healthy Japanese rabbits were randomly divided into the control and the experiment groups. In the experiment group, ANFH was induced by injection of horse serum and a high dose of corticosteroid. In the control group, normal saline was injection with the same volume. Contents of total cholesterol (TC) and triacylglycerol (TG) of the blood serum were measured at 2, 4, 6 and 8 weeks after injection. Animals were sacrificed at 2, 4 and 6 weeks and the pathological changes were detected with hematoxylin-eosin staining. Digital radiography (DR) and computed tomography (CT) were performed at 8 weeks before killing the remaining rabbits.
RESULTS AND CONCLUSION: In experiment group: DR showed bilaterally decreased bone density, an unclear epiphyseal line, and blurred texture of cancellous bone. CT showed spot-like low-density imaging of cancellous bone, thinner cortical bone, osteoporosis, and an unclear epiphyseal line. Hematoxylin-eosin staining of pathological sections showed fewer trabeculae and thin bone, an increased proportion of empty osteocyte lacunae, decreased hematopoiesis, thrombosis, and fat cell hypertrophy. No obviously change was found in the control group. The contents of TC and TG in experiment group were increased after model preparation (P < 0.05), which was apparently greater than that of the control group (P < 0.01). Rabbit model of ANFH can be successfully established by combination of horse serum and corticosteroid. Metabolic disorder of fatty substance is a very important pathogenesis of steroid-induced avascular necrosis of femoral head.

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