中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (27): 5119-5122.doi: 10.3969/j.issn.1673-8225.2010.27.042

• 干细胞学术探讨 stem cell academic discussion • 上一篇    下一篇

嗅鞘细胞移植治疗脊髓损伤的临床验证

郑遵成1，魏开斌1，刘 峰1，刘 超2，魏树刚2，程 宗3，高 瑞2，张 磊2， 张 昆2，匡乃峰2 ，张立清2   

  1. 1泰安中心医院骨科，山东省泰安市  271000；2泰安荣军医院脊柱脊髓科，山东省泰安271000；3泰山医学院统计学教研室，山东省泰安市  271000
  • 出版日期:2010-07-02 发布日期:2010-07-02
  • 作者简介:郑遵成★，男，1965年生，山东省汶上县人，汉族，2005年潍坊医学院毕业，硕士，副主任医师，主要从事细胞移植治疗中枢神经损伤研究。zhengzuncheng65@tom.com

Clinical verification of olfactory ensheathing cell transplantation in treatment of spinal cord injury

Zheng Zun-cheng1, Wei Kai-bin1, Liu Feng1, Liu Chao2, Wei Shu-gang2, Cheng Zong3, Gao Rui2,  Zhang Lei2, Zhang Kun2, Kuang Nai-feng2, Zhang Li-qing2   

  1. 1 Department of Orthopaedics, Taian Central Hospital, Taian   271000, Shandong Province, China; 2 Department of Spine and Spinal Cord, Taian Disabled Soldiers Hospital, Taian   271000, Shandong Province, China; 3 Department of Statistics, Taishan Medical College, Taian   271000, Shandong Province, China
  • Online:2010-07-02 Published:2010-07-02
  • About author:Zheng Zun-cheng★, Master, Associate chief physician, Department of Orthopaedics, Taian Central Hospital, Taian 271000, Shandong Province, China zhengzuncheng65@tom.com

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485

被引次数

26

摘要：

背景: 一系列基础研究证实在动物脊髓损伤的模型中，嗅鞘细胞移植能够促进脊髓损伤的再生和恢复脊髓的部分神经功能。部分临床实验证明嗅鞘细胞的移植的确能改善脊髓损伤患者的神经功能，从而改善其生活质量。
目的：验证嗅鞘细胞移植对脊髓损伤患者神经功能修复的作用及安全性。
方法：取流产胚胎嗅球并消化成为单个嗅鞘细胞，培养纯化2周左右，制成嗅鞘细胞悬液。选择脊髓损伤患者213例，全麻下将制备好的嗅鞘细胞悬液采用区域性多靶点注射方法移植于损伤脊髓的周围。采用ASIA量表对患者移植前1d及移植后3周~2个月进行评分，评价患者脊髓恢复状况。
结果与结论：所有患者的脊髓神经功能于术后3周均有不同程度的改变，脊髓功能评分及感觉与运动功能均较移植前明显提高(P < 0.001)，且随时间延长呈不断改善趋势；最长患者随访5年，未见已恢复的神经功能 减退及移植不良反应。证实嗅鞘细胞移植对脊髓损伤患者的神经功能恢复有促进作用，可以部分恢复及改善其部分脊髓神经功能，且治疗方法安全。

关键词: 脊髓损伤, 功能恢复, 神经功能, 移植, 嗅鞘细胞

Abstract:

BACKGROUND: A series of basic researches have confirmed that, the olfactory ensheathing cell transplantation can promote spinal cord regeneration and recover some neurological functions of spinal cord in animal models of spinal cord injury. Some clinical trials also prove that transplantation of olfactory ensheathing cells can indeed improve neurological function in patients with spinal cord injury, and then improve their quality of life.
OBJECTIVE: To verify the effectiveness and safety of olfactory ensheathing cell transplantation in repair of neurological function of spinal cord injury patients.
METHODS: The aborted embryonic olfactory bulb was collected and digested into single olfactory ensheathing cells. After they were cultured and purified 2 weeks, olfactory ensheathing cell suspension was prepared. A total of 213 cases of spinal cord injury were selected. Under general anesthesia, the prepared olfactory ensheathing cell suspension was injected through several target sites surrounding the injured spinal cord. ASIA scale was used to assay the patients before transplantation, 3 weeks to 2 months after transplantation, so as to evaluate spinal cord recovery.
RESULTS AND CONCLUSION: The spinal cord nerve function in all patients altered to different degrees at 3 weeks postoperation. Spinal cord function score, the sensory and motor functions were significantly increased compared with preoperation (P < 0.001), and showed a trend of continuous improvement with time; the patients were visited as follow-up for no more than 5 years, and no impairment of the restored nervous function or transplant adverse reactions were observed. It is confirmed that olfactory ensheathing cell transplantation can promote the recovery of nerve function in patients with spinal cord injury, it can restore and improve some spinal cord functions, and the treatment is safe.

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