中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (24): 4390-4392.doi: 10.3969/j.issn.1673-8225.2010.24.005

• 骨组织构建 bone tissue construction • 上一篇    下一篇

雌激素对成骨细胞血管内皮生长因子表达的影响

潘晋平，尹芸生，李鹏彪，闻伟敬   

  1. 山西医科大学第二临床医院骨科，山西省太原市 030001
  • 出版日期:2010-06-11 发布日期:2010-06-11
  • 通讯作者: 尹芸生，主任医师，硕士生导师，山西医科大学第二临床医院骨科，山西省太原市 030001 yunshengyin@ hotmail.com
  • 作者简介:潘晋平★，男，1982年生，山西省晋城市人，汉族，山西医科大学在读硕士，主要从事骨质疏松的骨愈合机制的研究。 ibmapple33@163.com

Effect of estrogen on vascular endothelial growth factor expression in rat osteoblasts

Pan Jin-ping, Yin Yun-sheng, Li Peng-biao, Wen Wei-jing   

  1. Department of Orthopaedics, Second Clinical Hospital, Shanxi Medical University, Taiyuan   030001, Shanxi Province, China
  • Online:2010-06-11 Published:2010-06-11
  • Contact: Yin Yun-sheng, Chief physician, Master’s supervisor, Department of Orthopaedics, Second Clinical Hospital, Shanxi Medical University, Taiyuan 030001, Shanxi Province, China yunshengyin@hotmail.com
  • About author:Pan Jin-ping★, Studying for master’s degree, Department of Orthopaedics, Second Clinical Hospital, Shanxi Medical University, Taiyuan 030001, Shanxi Province, China ibmapple33@163.com

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摘要：

背景：在众多的血管生长因子中，血管内皮生长因子是最有力的血管生成因子，它具有促进血管内皮细胞分裂、增殖，细胞质钙化聚集并能诱导血管生成等作用，且对于成骨细胞、破骨细胞的增殖、分化及功能活性具有重要调节作用。
目的：观察雌激素对成骨细胞上血管内皮生长因子表达的影响。
方法：取新生48 h大鼠颅盖骨，酶解消化得到成骨细胞，并进行体外培养。采用RT-PCR法测定10-10，10-8，10-6，10-4 mol/L 17β-雌二醇作用成骨细胞后血管内皮生长因子mRNA的表达。
结果与结论：RT-PCR检测结果显示，血管内皮生长因子mRNA在原代成骨细胞内稳定表达；半定量分析证实，不同浓度17β-雌二醇组间血管内皮生长因子mRNA的表达量呈现一定规律，随着17β-雌二醇浓度的升高，血管内皮生长因子mRNA的表达量逐渐增加，10-6 mol/L左右时，血管内皮生长因子mRNA的表达量最高，超过此剂量，血管内皮生长因子的表达下降。提示雌激素促进成骨细胞上血管内皮生长因子的表达上调，并存在剂量依赖性。

关键词: 雌激素, 血管内皮生长因子, 成骨细胞, 细胞培养, 骨组织工程

Abstract:

BACKGROUND: Vascular endothelial growth factor (VEGF) is a major angiogenesis factor, characterizing by promoting cell division, proliferation, cytoplasm calcification and aggregation, as well as inducing angiogenesis; on the other hand, it also adjusts proliferation, differentiation, and functional activity of osteoblast and osteoclast.
OBJECTIVE: To investigate the effect of estrogen on VEGF expression in rat osteoblasts.
METHODS: Calvaria osteoblasts derived from 48-hour-old rats were cultured to obtain osteoblast. RT-PCR was employed to detect the VEGF mRNA expression following induction of 17β-estradiol at concentrations of 10-10, 10-8, 10-6, and 10-4 mol/L.
RESULTS AND CONCLUSION: RT-PCR demonstrated that VEGF mRNA was stably expressed in primary cultured osteoblast. Semi-quantitative analysis demonstrated that VEGF mRNA expression was in a certain law following 17β-estradiol induction at varying concentrations, i.e., with the increasing concentration of 17β-estradiol, VEGF mRNA expression was increased. At 10-6 mol/L, the VEGF mRNA expression peaked, and when 17β-estradiol was over this dose, the expression was gradually decreased. This suggested that estrogen promoted VEGF mRNA expression, showing a dose-dependent manner.

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