中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (23): 4203-4206.doi: 10.3969/j.issn.1673-8225.2011.23.006

• 骨髓干细胞 bone marrow stem cells • 上一篇    下一篇

心肌条件培养液与5-氮胞苷诱导骨髓基质干细胞向心肌样细胞的分化

王  洋1，沈慧娟2，周敏芬1，吴彬俏1，曾宪智2   

  1. 嘉兴学院，1医学院临床医学系；2机电工程实验中心；3 医学院解剖学教研室，浙江省嘉兴市  314001
  • 收稿日期:2010-12-13 修回日期:2011-04-25 出版日期:2011-06-04 发布日期:2011-06-04
  • 通讯作者: 曾宪智，博士，讲师，嘉兴学院医学院解剖学教研室，浙江省嘉兴市314001 frankzengxzh@yahoo.cn
  • 作者简介:王洋，男，1988年生，浙江省嘉兴市人，汉族，嘉兴学院在读学生，主要从事医学方面的研究。 wydc88@sina.com
  • 基金资助:

    浙江省大学生科技创新活动计划项目(2009R417002)，骨髓基质干细胞的分离培养及定向诱导为肌肉样细胞的研究。

Induction of marrow stromal cells into myocardical cells by conditioned cultrure medium of cardiac muscle and 5-azacytidine

Wang Yang1, Shen Hui-juan2, Zhou Min-fen1, Wu Bin-qiao1, Zeng Xian-zhi2   

  1. 1Department of Clinical Medicine, College of Medicine, 2Laboratory Center of Engineering of  Machinery and Electronics, 3Department of Anatomy, College of Medicine, Jiaxing University, Jiaxing  314004, Zhejiang Province, China
  • Received:2010-12-13 Revised:2011-04-25 Online:2011-06-04 Published:2011-06-04
  • Contact: Zeng Xian-zhi, Doctor, Lecturer, Laboratory Center of Engineering of Machinery and Electronics, Jiaxing University, Jiaxing 314004, Zhejiang Province, China frankzengxzh@yahoo.cn
  • About author:Wang Yang, Department of Clinical Medicine, College of Medicine, Jiaxing University, Jiaxing 314004, Zhejiang Province, China wydc88@sina.com
  • Supported by:

    the Science and Technology Innovation Program of Undergraduate Students in Zhejiang Province, No. 2009R417002*

PDF

289

被引次数

9

摘要：

背景：骨髓基质干细胞可以明显改善心肌缺血时的心脏功能，但其直接分化为心肌细胞并参与心功能恢复的机制尚不明确。
目的：探讨心肌条件培养液与5-氮胞苷诱导骨髓基质干细胞分化为心肌样细胞的作用。
方法：全骨髓贴壁法分离培养骨髓基质干细胞，将第6代骨髓基质干细胞随机分为4组：5-氮胞苷+心肌条件培养液联合诱导组；5-氮胞苷组；心肌条件培养液组；基础培养基组(空白组)。用心肌条件培养液和5-氮胞苷诱导3周后用免疫组化法测定各组心肌肌钙蛋白表达，并采用单因素方差分析检验进行统计学分析。
结果与结论：在体外心肌条件培养液可以诱导骨髓基质干细胞向心肌细胞分化，提示其中细胞因子可能起关键作用，但心肌条件培养液的这种作用要弱于化学诱导剂5-氮胞苷的诱导作用，且联合诱导作用更强。

关键词: 骨髓基质干细胞, 细胞培养, 心肌细胞, 诱导, 分化

Abstract:

BACKGROUND: Bone marrow stromal cells (MSCs) can significantly improve cardiac function after ischemia-induced myocardium injury, but the mechanism of differentiation of MSCs into myocardical cells and its contribution to the regeneration of cardiac function remains uncertain.
OBJECTIVE: To explore the effects of conditioned cultrure medium of cardiac muscle and 5-azacytidine on inducing MSCs into myocardial cells.
METHODS: MSCs were sorted from whole bone marrow by adherent culture. MSCs of the 6th generation were divided into 4 groups: combined group (5-azacytidine + conditioned cultrure medium of cardiac muscle; 5-azacytidine group; conditioned cultrure medium group; blank group (basal culture medium). At 3 weeks after induction by 5-azacytidine and conditioned cultrure medium of cardiac muscle, the expression of myocardial troponin was measured by immunohistochemistry and analysed by one-way analysis of variance.
RESULTS AND CONCLUSION: MSCs could be induced into myocardial cells by conditioned cultrure medium of cardiac muscle in vitro, indicating that the cytokines in the cultrure medium might play key roles in the induction. But such effect of conditioned cultrure medium of cardiac muscle was weaker than that of 5-azacytidine. The effect of combined induction was the strongest. 

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