中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (18): 3323-3327.doi: 10.3969/j.issn.1673-8225.2011.18.023

• 移植与免疫 transplantation and Immunology • 上一篇    下一篇

内皮微粒在肾移植急性排斥反应中的诊断作用

杨  静1，崔节伟1，李定宪2，曹伟科1，孙  奕1   

  1. 1武警医学院免疫学教研室，天津市 300162
    2武警江苏总队常州支队卫生队，江苏省常州市213003
  • 收稿日期:2010-10-22 修回日期:2011-01-10 出版日期:2011-04-30 发布日期:2011-04-30
  • 通讯作者: 孙奕，博士，教授，武警医学院免疫学教研室，天津市300162 drsunyi@163.com
  • 作者简介:杨静★，女，1976年生，天津市人，汉族，2006年天津医科大学毕业，硕士，讲师，主要从事内皮细胞损伤与排斥反应的关系方面的研究。 yangjingdj@hotmail.com
  • 基金资助:

    天津市应用基础及前沿技术计划面上项目(08JCYBJC06800)，课题名称：内皮微粒与肾移植急性排斥反应相关性的实验研究。武警医学院面上项目(WY200912)，课题名称；肾脏移植中内皮微粒的数量和表型与急性排斥反应相关性研究。

Endothelial microparticles for diagnosis of acute rejection following renal transplantation

Yang Jing1, Cui Jie-wei1, Li Ding-xian2, Cao Wei-ke1, Sun Yi1   

  1. 1Department of Immunology, Medical College of Chinese People’s Armed Police Force, Tianjin  300162, China
    2Health Team, Changzhou Detachment of Jiangsu General Armed Forces, Changzhou  213003, Jiangsu Province, China
  • Received:2010-10-22 Revised:2011-01-10 Online:2011-04-30 Published:2011-04-30
  • Contact: Sun Yi, Doctor, Professor, Department of Immunology, Medical College of Chinese People’s Armed Police Force, Tianjin 300162, China drsunyi@163.com
  • About author:Yang Jing★, Master, Lecturer, Department of Immunology, Medical College of Chinese People’s Armed Police Force, Tianjin 300162, China yangjingdj@hotmail.com
  • Supported by:

    the Applied Basis and Advanced Techonlogy Program of Tianjin, No. 08JCYBJC06800*; the General Program of Medical College of Chinese People’s Armed Police Force, No. WY200912*

PDF

415

被引次数

2

摘要：

背景：血管内皮细胞的变化与移植排斥反应的关系极为密切，内皮微粒脱落于激活或凋亡的内皮细胞，能直接而特异地反映血管内皮细胞的变化，检测血浆中内皮微粒对肾移植排斥反应的诊断监测具有一定的理论和实际意义。
目的：探讨肾移植急性排斥反应时循环内皮微粒的数量和表型的变化及与急性排斥反应之间的关系。
方法：建立同基因和同种异基因大鼠腹腔原位肾移植模型；移植后5 d苏木精-伊红染色观察肾组织的病理学改变，并进行Banff评分；采用免疫组化法检测肾组织中细胞间黏附分子1表达；采用流式细胞术检测血浆中CD144+内皮微粒数量及细胞间黏附分子1+/CD144+内皮微粒的数量；分析内皮微粒的数量和表型与肾组织病理变化的关系。
结果与结论：与同基因移植组比较，异基因移植组Bnaff评分明显增加(P < 0.01)，肾组织中细胞间黏附分子1表达明显增强(P < 0.01)；与同基因移植组比较，异基因移植组CD144+内皮微粒的数量和携带细胞间黏附分子1的内皮微粒的水平明显增加(P < 0.01)。内皮微粒的数量与移植肾急性排斥反应的程度呈正相关(P < 0.01)，携带细胞间黏附分子1内皮微粒的水平与移植肾脏中细胞间黏附分子1的表达呈正相关(P < 0.01)。提示肾移植后对内皮微粒数量和表型进行检测对诊断急性排斥反应的发生有一定意义。

关键词: 内皮微粒, 肾移植, 异基因移植, 急性排斥反应, 细胞间黏附分子1

Abstract:

BACKGROUND: Variation of endothelial cells is closely associated with graft rejective reaction. After transplantation, endothelial microparticles (EMPs) are shed from the plasma membrane of endothelial cells, and can reflect the change of endothelial cells directly. Accordingly, it is significant to detect plasma EMPs for monitoring acute rejection after renal transplantation.   
OBJECTIVE: To study the changes of EMPs in rats of acute rejection after renal transplantation and their relations with acute rejection.
METHODS: The syngeneic and allogeneic rat models of orthotopic renal transplantation were established. Pathological changes of the kidneys were observed at 5 days after transplantation by haematoxylin-eosin staining, and were graded according to the Bnaff criteria. The expression of intercellular adhesion molecule-1 in rat kidneys was quantitatively detected by immunohistochemistry. The level of CD144+ EMPs and intercellular adhesion molecule (ICAM)-1+/CD144+ EMPs were measured by flow cytometry. The relation between quantity and phenotype of EMPs and pathological changes of kidneys was analyzed.
RESULTS AND CONCLUSION: Compared with syngeneic group, the Bnaffs grades (P < 0.01), the expression of ICAM-1 of kidney (P < 0.01) in allogeneic group was significantly increased. Compared with syngeneic group, the level of CD144+ EMPs and ICAM-1+/CD144+EMPs (P < 0.01) in allogeneic group were significantly increased. The level of CD144+ EMPs was positively related with the Bnaffs grades (P < 0.01), and the level of ICAM-1+/ CD144+EMPs was positively related with the expression of ICAM-1 of kidney (P < 0.01). The level of EMPs detection may be helpful to the diagnosis of acute rejection after transplantation.

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