中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (18): 3288-3292.doi: 10.3969/j.issn.1673-8225.2011.18.015

• 心肺移植 heart-lung transplantation • 上一篇    下一篇

异位气管移植模型中上皮细胞的增殖与凋亡

王林毛1，2，吴  凯3，高  文2，陈  昶2，鲍  方2，郑  卉2   

  1. 1苏州大学医学院， 江苏省苏州市  215000
    2同济大学医学院附属上海市肺科医院胸外科，上海市  200433
    3山东省沂源县人民医院普外科，山东省淄博市  256100
  • 收稿日期:2010-10-13 修回日期:2010-11-06 出版日期:2011-04-30 发布日期:2011-04-30
  • 通讯作者: 高文，教授，主任医师，同济大学附属上海市肺科医院胸外科，上海市200433 gaowen5921@163.com
  • 作者简介:王林毛★，男，1984年生，汉族，江苏省盐城市人，苏州大学在读硕士，主要从事肺移植的基础和临床研究。 wanglinmao12345@sina.com 并列第一作者：吴凯★，男，1983年生，汉族，山东省淄博市人，2010年苏州大学毕业，硕士，主要从事肺移植的基础和临床研究。

Epithelial proliferation and apoptosis in heterotopic tracheal transplantation model

Wang Lin-mao1,2, Wu Kai3, Gao Wen2, Chen Chang2, Bao Fang2, Zheng Hui2   

  1. 1Medical College of Soochow University, Suzhou  215000, Jiangsu Province, China
    2Department of Thoracic Surgery, Affiliated Shanghai Pulmonary Hospital, Medical College of Tongji University, Shanghai   200433, China
    3Department of General Surgery, Yiyuan Country People’s Hospital, Zibo   256100, Shandong Province, China
  • Received:2010-10-13 Revised:2010-11-06 Online:2011-04-30 Published:2011-04-30
  • Contact: Gao Wen, Professor, Chief physician, Department of Thoracic Surgery, Affiliated Shanghai Pulmonary Hospital, Medical College of Tongji University, Shanghai 200433, China gaowen5921@163.com
  • About author:Wang Lin-mao★, Studying for master’s degree, Medical College of Soochow University, Suzhou 215000, Jiangsu Province, China; Department of Thoracic Surgery, Affiliated Shanghai Pulmonary Hospital, Medical College of Tongji University, Shanghai 200433, China wanglinmao12345@sina.com Wu Kai, Master, Department of General Surgery, Yiyuan Country People’s Hospital, Zibo 256100, Shandong Province, China Wang Lin-mao and Wu Kai contributed equally to this paper

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276

被引次数

5

摘要：

背景：异位气管移植中上皮细胞的变化过程尚不清楚，有研究表明在上皮增殖同时伴随有不可逆的细胞死亡，并且气道上皮再生与死亡发生在移植气道闭塞之前。
目的：观察异位鼠气管移植过程中气道上皮细胞的增殖与凋亡情况。
方法：将异体BALB/c小鼠气管植入BALB/c小鼠颈背部皮下，移植后不注射环孢素A。将BALB/c小鼠气管植入C57BL/6小鼠颈背部皮下，一组不注射环孢素A，另一组全程腹腔注射环孢素A。移植后第3，7，14，21，30天苏木精-伊红染色评价气道上皮的完整性、黏膜下炎症细胞浸润和纤维组织增生情况；测定移植气管中BrdU标记指数及TUNEL阳性细胞数。
结果与结论：受体BALB/c小鼠移植后第3天气道上皮细胞轻度损伤丢失，第7~21天，气道上皮逐渐恢复正常，第30天，气道上皮接近正常上皮；BrdU标记指数变化范围为3%~5%；TUNEL阳性细胞数基本无变化。受体C57BL/6小鼠移植后第3天，气道上皮轻度损伤，第7~21天，气管上皮脱落、坏死，开始出现纤维增殖并逐渐加重，第30天时，上皮细胞完全消失，管腔被纤维组织填塞，接近闭塞；移植后第14天，BrdU标记指数达到最高，未注射环孢素A组高于注射环孢素A组(P=0.000)，移植后第21，30天，标记指数明显降低；移植后第21天，未注射环孢素A组TUNEL阳性细胞数达到最大值，注射环孢素A组于移植后第14天达最大值。说明异位气管移植上皮增殖同时伴随有不可逆的细胞凋亡，环孢素A可以减轻闭塞性细支气管炎的发病程度，但并不能阻止其发生。

关键词: 气管移植, 同种异体移植, 闭塞性气道疾病, 同系移植, 环孢素A, 器官移植

Abstract:

BACKGROUND: The process of epithelial cells in heterotopic trachea transplantation remains unclear. The studies demonstrated that epithelial proliferation is accompanied by irreversible cell death, and airway epithelial regeneration and death occurred before airway occlusion transplantation.
OBJECTIVE: To observe epithelial proliferation and apoptosis in the process of heterotopic mouse trachea transplantation.
METHODS: The allogeneic BALB/c mouse trachea was implanted subcutaneously in the nape of BALB/c mouse. Ciclosporin A (CsA) was not injected after transplantation. The allogeneic BALB/c mouse trachea was implanted subcutaneously in the nape of C57BL/6 mouse. One group was not injected with CsA, the other group was the whole course injected with CsA in abdominal cavity. At 3, 7, 14, 21, 30 days after transplantation, the integrity of airway epithelium, submucosal infiltration of inflammatory cells and fibrous tissue proliferation were evaluated by hematoxylin-eosin staining. BrdU labelling index and TUNEL positive cells in trachea transplantation were determined.
RESULTS AND CONCLUSION: At 3 days after receptor BALB/c mouse transplantation, airway epithelial cells suffered from minor injury and loss. At 7-21 days after transplantation, airway epithelial cells recovered normal gradually. Airway epithelium approached to normal epithelium at 30 days after transplantation. The extent of BrdU labelling index was 3%- 5%. The number of TUNEL positive cells was basically unchanged. At 3 days after receptor C57BL/6 mouse transplantation, airway epithelial cells suffered from minor injury. The shedding and necrosis were existed in airway epithelium, at 7-21 days after transplantation; fibrous proliferation was appeared and became more serious gradually. At 30 days after transplantation, epithelial cells completely disappeared, lumens were filled by fibrous tissue, closed to occlusion. The peak of BrdU labelling index was at 14 days after transplantation. The group of not injected with CsA was higher than group of injected with CsA in BrdU labelling indexes (P=0.000). At 21 and 30 days after transplantation, the BrdU labelling index was significantly reduced. The peak of UNEL positive cells in group of not injected with CsA was at 21 days after transplantation, the peak of UNEL positive cells group of injected with CsA was at 14 days after transplantation. It is indicated that epithelial proliferation in heterotopic trachea transplantation was accompanied by irreversible cell death. CsA could reduce the incidence of bronchiolitis obliterans, but did not prevent its occurrence.

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