中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (28): 5317-5320.doi: 10.3969/j.issn.1673-8225.2010.28.045

• 组织构建临床实践 clinical practice in tissue construction • 上一篇    下一篇

骨钙素基因Hind Ⅲ多态性与体质量指数变异的相关联性：南昌地区绝经前女性390例调查

徐  宏1，杨玉瓶2，刘永明3，涂云明2，彭  婧1，张  莉1，邹  琳4，况海斌1   

  1. 1南昌大学医学院生理学教研室，江西省南昌市   330006; 2南昌大学第四附属医院肾脏和内分泌科，江西省南昌市   330006；3江西省心理康复中心心理科，江西省南昌市  330006；4江西省人民医院放免科，江西省南昌市   330006
  • 出版日期:2010-07-09 发布日期:2010-07-09
  • 通讯作者: 况海斌，博士，副教授，南昌大学医学院生理学教研室，江西省南昌市330006
  • 作者简介:徐 宏★，女，1978年生，江西省南昌市人，汉族，2005年湖南师范大学毕业，硕士，讲师，主要从事内分泌学的基础研究。
  • 基金资助:

    课题受国家自然科学基金(30760071、30960118)，江西省教育厅基金(GJJ08106)和南昌大学科研课题基金(J06)资助。

Association between osteocalcin Hind Ⅲ genetic polymorphism and body mass index variation
Investigation of 390 premenopausal women in Nanchang region

Xu Hong1, Yang Yu-ping2, Liu Yong-ming3, Tu Yun-ming2, Peng Jing1, Zhang Li1, Zou Lin4, Kuang Hai-bin1   

  1. 1 Department of Physiology, School of Medicine, Nanchang University, Nanchang  330006, Jiangxi Province, China; 2 Department of Nephrology and Endocrinology, The Fourth Affiliated Hospital of Nanchang University, Nanchang  330006, Jiangxi Province, China; 3 Department of Psychology, Center of Psychological Rehabilitation, Nanchang  330006, Jiangxi Province, China; 4 Department of Radioimmunology, People’s Hospital of Jiangxi Province, Nanchang  330006, Jiangxi Province, China
  • Online:2010-07-09 Published:2010-07-09
  • Contact: Kuang Hai-bin, Doctor, Associate professor, Department of Physiology, School of Medicine, Nanchang University, Nanchang 330006, Jiangxi Province, China kuanghaibin@ncu.edu.cn
  • About author:Xu Hong★, Master, Lecturer, Department of Physiology, School of Medicine, Nanchang University, Nanchang 330006, Jiangxi Province, China Hongxu1978@yahoo.com.cn
  • Supported by:

    the National Natural Science Foundation of China, No. 30760071*, 30960118*; Foundation of the Education Department of Jiangxi Province, No. GJJ08106*; Science and Technology Research Program of Nanchang University, No. J06*

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摘要：

背景： 体质量指数(BMI)是研究肥胖的常用表型，它是由多基因决定的复杂性状。
目的：验证骨钙素基因Hind Ⅲ多态性是否与体质量指数的变异相关联。
方法：征集南昌当地绝经前女性390例，并测量身高和体质量。用聚合酶链式反应-限制性片段长度多态性(PCR-RFLP)法对所有个体的骨钙素基因Hind Ⅲ位点进行基因分型。
结果与结论：骨钙素HH，Hh和hh基因型频率分别为0.077，0.408和0.515。骨钙素基因型分布符合哈的温格尔平衡(P > 0.05)。骨钙素基因Hind Ⅲ与体质量指数存在显著的关联(P=0.002)，它可以解释大约5.47%体质量指数的变异。HH基因型个体的体质量指数最高[(22.81±0.73)kg/m2]，Hh基因型个体的体质量指数居中[(21.50±0.53) kg/m2]，而hh基因型个体的体质量指数最低[(20.23± 0.63) kg/m2]。因此，HH和Hh基因型个体的体质量指数比hh基因型个体的体质量指数分别高大约12.75%和6.28%。文章首次在健康绝经前女性中报道骨钙素基因HindⅢ多态性与体质量指数的变异相关联。

关键词: 骨钙素基因, Hind Ⅲ, 多态性, 体质量指数, 关联

Abstract:

BACKGROUND: Body mass index (BMI) is a commonly used phenotype for obesity, which is determined by multiple genetic factors.
OBJECTIVE: To investigate whether osteocalcin (also known as bone Gla protein, BGP) Hind Ⅲ genetic polymorphism is associated with BMI variation.
METHODS: A total of 390 premenopausal women from a local population of Nanchang City were selected. Body weight and height were measured. All participants were genotyped at the BGP Hind Ⅲ locus using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).
RESULTS AND CONCLUSION: The BGP genotype frequencies of HH, Hh and hh were 0.077, 0.408 and 0.515, respectively. The distribution of BGP Hind Ⅲ genotypes was consistent with Hardy-Weinberg equilibrium (P > 0.05). The BGP Hind Ⅲ were significantly associated with BMI (P=0.002), which could explain about 5.47% of BMI variation. On average, BMI of individuals with HH genotype was the highest [(22.81±0.73) kg/m2], individuals with Hh genotype was intermediate [(21.50±0.53) kg/m2], while individuals with hh genotype was the lowest [(20.23±0.63) kg/m2]. Therefore, carriers of HH and Hh genotypes had, respectively, approximately 12.75% and 6.28% higher BMI than carriers of the hh genotype. To our best knowledge, this is the first study reporting the association of BGP Hind Ⅲ genetic polymorphism and BMI in healthy premenopausal women.

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