中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (16): 2851-2854.doi: 10.3969/j.issn.1673-8225.2010.16.001

• 组织工程骨及软骨材料 tissue-engineered bone and cartilage materials •    下一篇

个性化钛模板与纳米羟基磷灰石及自体骨修复兔上颌骨缺损

王稚英，李世德，马晓凛，赵冰净，金  鼎，葛一鸣，亓  峰   

  1. 辽宁医学院附属第二医院暨附属口腔医院口腔颌面外科，辽宁省锦州市 121001
  • 出版日期:2010-04-16 发布日期:2010-04-16
  • 作者简介: 王稚英☆，男，1964年生，辽宁省锦州市人，汉族，2004年北京大学医学部毕业，博士，教授，主任医师，主要从事口腔种植与牙颌功能重建的研究。 bdwzy@126.com
  • 基金资助:

    辽宁省自然科学基金(20092186)，项目名称：个性化钛模板作为组织工程外支架修复牙种植体周围骨量不足的实验研究。辽宁省教育厅高等学校科学研究项目(05L138)，项目名称：个性化钛网、钛板联合重建中空上颌骨的临床和基础研究。

Effect of personalized prefabricated titanium template, autologous bone and nano-hydroxyapatite on reconstruction of maxilla defect in rabbits

Wang Zhi-ying, Li Shi-de, Ma Xiao-lin, Zhao Bing-jing, Jin Ding, Ge Yi-ming, Qi Feng   

  1. Department of Oral Maxillofacial Surgery, Second Affiliated Hospital and Affiliated Stomatological Hospital, Liaoning Medical University, Jinzhou  121001, Liaoning Province, China
  • Online:2010-04-16 Published:2010-04-16
  • About author:Wang Zhi-ying☆, Doctor, Professor, Chief physician, Department of Oral Maxillofacial Surgery, Second Affiliated Hospital and Affiliated Stomatological Hospital, Liaoning Medical University, Jinzhou 121001, Liaoning Province, China Bdwzy@126.com
  • Supported by:

    the Natural Science Foundation of Liaoning Province, No. 20092186; Scientific Research Program of Liaoning Department of Education, No. 05L138*

PDF

706

被引次数

12

摘要：

背景：自体骨、骨替代材料及骨诱导再生技术可修复颌骨缺损，但由于新骨形成周期长、骨替代材料及骨诱导再生膜吸收快，缺损区新骨的体积和塑形受到影响。
目的：探讨个性化钛模板与纳米羟基磷灰石及自体骨在兔上颌骨牙槽嵴缺损修复中的作用。
方法：18只日本大耳白兔，随机分为2组，制作长10 mm，高5 mm的上颌牙槽嵴骨缺损，实验组骨缺损区由自体髂骨颗粒及缺损区制作中产生的自体骨颗粒与纳米羟基磷灰石填充，表面覆盖个性化钛模板，并螺钉固定；对照组缺损区只覆盖个性化钛模板并螺钉固定，缺损区不充填任何骨移植材料。观察术后4，8，12周的新骨生成情况、X射线表现及苏木精-伊红染色组织学改变。
结果与结论：术后4周时，实验组新骨形成明显优于对照组；术后8及12周时，两组形成的新骨无明显区别。经配对t检验，实验组和对照组在术后4周时新骨密度差异有显著性意义(P < 0.01)，而在术后8周和12周时，新骨灰度值差异无显著性意义(P > 0.05)。提示自体骨及纳米羟基磷灰石可很好地修复上颌牙槽嵴缺损；个性化钛模板可起到屏障膜和新骨形成外支架的作用，有利于新骨的塑形。

关键词: 上颌骨缺损, 自体骨, 纳米羟基磷灰石, 个性化钛模板, 口腔生物材料

Abstract:

BACKGROUND: Autologous bone, bone substitute materials and guided bone regeneration (GBR) technique can repair jaw defects, but the absorption speed of bone substitute materials and GBR membrane are faster than the formation speed of new bone, therefore, it affects the volume and shape of new bone.
OBJECTIVE: To investigate the role of personalized prefabricated titanium template, autologous bone and nano-hydroxyapatite on restoration of maxillary defect in rabbit.
METHODS: A total of 18 rabbits were randomly divided into two groups, and maxillary alveolar defect with 10 mm length and    5 mm high was created. The template was implanted in both two groups, and fastened with titanium screws. Autologous and nano-hydroxyapatite were placed into the defect in experimental group; neither autologous bone nor bone substitute materials were implanted into the defect in control group. New bone formation, X-ray findings, and histological changes with HE stain were carried out 4, 8 and 12 weeks postoperatively.
RESULTS AND CONCLUSION: The quality of new bone in experimental group was batter than that in control group 4 weeks postoperatively, but the quality of new bone was almost the same 8 and 12 weeks postoperatively. By paired t-test, there was significant difference in new bone density between the experimental group and the control group 4 weeks after operation (P < 0.01), but there was no significant difference in new bone density between the experimental group and the control group 8 and 12 weeks after operation (P > 0.05). Autologous bone and nano-hydroxyapatite can restore the defect of maxillary alveola. Personalized prefabricated titanium template can play an important role of screen membrane and external scaffold in new bone formation, and remain shape of new bone.

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