中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (15): 2756-2760.doi: 10.3969/j.issn.1673-8225.2010.15.023

• 组织构建与生物活性因子 tissue construction and bioactive factors • 上一篇    下一篇

体外压力刺激对大鼠“足三里”穴筋膜组织成纤维细胞合成释放基质金属蛋白酶1、基质金属蛋白酶抑制剂1、前列腺素E2和胰岛素样生长因子1的影响

陈  波1，崔  瑾1，谢西梅1，李佳霖1，李小玉2   

  1. 1贵阳中医学院 针灸推拿系，贵州省贵阳市 550002；2四川大学华西口腔医学院生物医学工程教育部重点实验室，四川省成都市   600041
  • 出版日期:2010-04-09 发布日期:2010-04-09
  • 作者简介:陈 波☆，男，1974年生，贵州省贵阳市人，汉族，2005年成都中医药大学毕业，博士，副教授，主要从事经络腧穴的基础与运用研究。 chenbomed@yahoo.com.cn
  • 基金资助:

    课题受国家自然科学基金项目（30801490）和贵州省科技厅科技基金项目（黔科合J字2006[2063]号）的资助。

Effects of in vitro pressure stimulation to fascia tissue of zusanli (ST36) on synthesis and release of fibroblast matrix metalloproteinase-1, matrix metalloproteinase inhibitor-1, prostaglandin E2 and insulin-like growth factor-1 

Chen Bo1, Cui Jin1, Xie Xi-mei1, Li Jia-lin1, Li Xiao-yu2   

  1. 1 Department of Acupuncture and Massage, Guiyang College of Traditional Chinese Medicine, Guiyang  550002, Guizhou Province, China; 2 Key Laboratory of Oral Biomedical Engineering of Ministry of Education, West China College of Stomatology, Sichuan University, Chengdu  600041, Sichuan Province, China
  • Online:2010-04-09 Published:2010-04-09
  • About author:Chen Bo☆, Doctor, Associate professor, Department of Acupuncture and Massage, Guiyang College of Traditional Chinese Medicine, Guiyang 550002, Guizhou Province, China chenbomed@yahoo.com.cn
  • Supported by:

    the National Natural Science Foundation of China, No. 30801490*; Foundation of Science and Technology Commission of Guizhou Province, No. 2006[2063]*

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摘要：

背景：腧穴筋膜结缔组织成纤维细胞是针推治疗操作过程中的主要受力组织细胞之一，细胞生物学行为的调整对腧穴发挥治疗作用的功能关系密切。
目的：通过体外实验观察压力刺激对大鼠“足三里”穴筋膜组织成纤维细胞合成释放基质金属蛋白酶1、基质金属蛋白酶抑制剂1、前列腺素E2和胰岛素样生长因子1的影响。
方法：体外培养大鼠“足三里”穴筋膜组织成纤维细胞，随机分为空白对照组和压力刺激组。压力刺激组对细胞实施50kPa压力刺激，每次加力2 h，每8 h1次，共6次；空白对照组不加压。检测细胞外培养液中基质金属蛋白酶1、基质金属蛋白酶抑制剂1、前列腺素E2和胰岛素样生长因子1含量的变化，并计算基质金属蛋白酶1与基质金属蛋白酶抑制剂1比值的变化。
结果与结论： “足三里”穴筋膜组织成纤维细胞压力作用后，培养液中基质金属蛋白酶1、基质金属蛋白酶抑制剂1和前列腺素E2含量均增加(P < 0.05或0.01)，基质金属蛋白酶1与基质金属蛋白酶抑制剂1比值增大(P < 0.05)，胰岛素样生长因子1变化无显著性意义。结果证实，压力刺激对腧穴筋膜组织成纤维细胞基质金属蛋白酶1、基质金属蛋白酶抑制剂1和前列腺素E2合成释放的调节，以及对基质金属蛋白酶1与基质金属蛋白酶抑制剂1比值的提高，可能是腧穴接受针灸推拿治疗后发挥“疏经通脉”作用的细胞生物力学原理之一。

关键词: 针灸推拿, 压力刺激, 腧穴, 筋膜组织成纤维细胞, 疏经通脉, 基质金属蛋白酶1, 基质金属蛋白酶抑制剂1, 前列腺素E2, 胰岛素样生长因子1

Abstract:

BACKGROUND: Fibroblasts in acupoint ascia tissues are major forces during acupuncture and massage therapy, and the regulation of cell biological behavior is closely associated with treatment efficiency.
OBJECTIVE: To observe the effect of in vitro pressure stimulation to zusanli (ST36) fascia on synthesis and release of fibroblast matrix metalloproteinase-1 (MMP-1), matrix metalloproteinase inhibitor-1 (TIMP-1), prostaglandin E2 and insulin-like growth factor-1 (IGF-1).
METHODS: Fascia fibroblasts of zusanli (ST36) were cultured in vitro and randomly divided into the control and pressure groups. Cells in the pressure group were stimulated by 50 kPa initial pressure for 2 hours, with an 8-hour interval for 6 times. There were no pressures in the control group. The extracellular medium changes of MMP-1, TIMP-1, prostaglandin E2 and IGF-1 was detected, and the range MMP-1 and TIMP-1 ratio (MMP-1/TIMP-1) was calculated.
RESULTS AND CONCLUSION: After pressure stimulation on zusanli (ST36) fascia fibroblasts, the MMP-1, TIMP-1 and prostaglandin E2 levels were increased in culture medium (P < 0.05 or P < 0.01), MMP-1/TIMP-1 increased (P < 0.05), and the difference of IGF-1 had no statistically significance. The results demonstrated that, pressure stimulation on acupoints fascia fibroblasts has a positive regulation of MMP-1, TIMP-1 synthesis, as well as the improvement of MMP-1/TIMP-1. Maybe it is the mechanical cellular biological principle of acupoints acupuncture therapy of sujingtongmai.

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