5-Fluorouracil enriches cancer stem cells in tongue squamous cell carcinoma Tca8113

doi:10.3969/j.issn.2095-4344.2015.10.002

Abstract

Abstract:

BACKGROUND: Tumor cells are resistant to chemotherapeutic drugs, and drug resistance is closely correlated with tumor stem cells. Therefore, how to kill tumor stem cells will become the key to the treatment of oral squamous cell carcinoma.

OBJECTIVE: To study the effect of 5-fluorouracil on biological characteristics of tongue squamous cell carcinoma Tca8113 cells.

METHODS: Viability of Tca8113 cells treated with different concentrations of 5-fluorouracil was determined by cell counting kit-8, and the best drug concentration and time were screened for subsequent experiments. Tca8113 cells without 5-fluorouracil acted as control group. Then the cell cycle and percentage of the side population cells in Tca8113 cells were determined by flow cytometry. Scratch test was used to determine the migration ability of Tca8113 cells.

RESULTS AND CONCLUSION: Results from cell counting kit-8 showed that 5-fluorouracil inhibited the viability of Tca8113 cells positively in a time- and dose-dependent manner. Tca8113 cells under intervention with 50 mg/L 5-fluorouracil for 48 hours showed lowest cell viability. Flow cytometry results showed that in the experimental group, G₀/G₁ phase cells increased significantly compared with the control group (P=0.01), S phase cells decreased significantly compared with the control group (P=0.244), and G₂/M phase cells disappeared completely. After treatment with 5-fluorouracil, the percentage of side population cells was increased significantly (P=0.00). The scratch test showed that in the experimental group, the cells had better ability of wound healing than those in the control group. In conclusion, 5-fluorouracil can enrich the cancer stem cell population in Tca8113 cells.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: Neoplastic Stem Cells, Fluorouracil, Cell Cycle

CLC Number:

R394.2

Xu Wang, Ji Ping. 5-Fluorouracil enriches cancer stem cells in tongue squamous cell carcinoma Tca8113[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(10): 1482-1487.

Figures/Tables 5

References

[1]Chen YJ, Chang JT, Liao CT, et al. Head and neck cancer in the betel quid chewing area: recent advances in molecular carcinogenesis.Cancer Sci. 2008;99(8):1507-1514.
[2]韩文遒. 结肠癌干细胞的筛选及其耐药性研究[J].医学美学美容:中旬刊,2014,22(3):141-142.
[3]Reya T, Morrison SJ, Clarke MF,et al.Stem cells, cancer, and cancer stem cells.Nature. 2001;414(6859):105-111.
[4]Lapidot T, Sirard C, Vormoor J,et al.A cell initiating human acute myeloid leukaemia after transplantation into SCID mice. Nature. 1994;367(6464):645-648.
[5]Dalerba P, Cho RW, Clarke MF.Cancer stem cells: models and concepts.Annu Rev Med. 2007;58:267-284.
[6]Wei XD, Zhou L, Cheng L,et al. In vivo investigation of CD133 as a putative marker of cancer stem cells in Hep-2 cell line. Head Neck. 2009;31(1):94-101.
[7]Clay MR, Tabor M, Owen JH,et al.Single-marker identification of head and neck squamous cell carcinoma cancer stem cells with aldehyde dehydrogenase.Head Neck. 2010;32(9):1195-1201.
[8]Tabor MH, Clay MR, Owen JH,et al.Head and neck cancer stem cells: the side population.Laryngoscope. 2011;121(3): 527-533.
[9]孙守娟,季平,邹波,等.无血清悬浮培养舌鳞癌Tca8113细胞系及肿瘤干细胞样亚群筛选[J].重庆医科大学学报,2011,36(12): 1416-1419.
[10]袁吉,倪孟祥.5-氟尿嘧啶及其衍生物的研究进展[J].药物生物技术,2012,19(5):462-465.
[11]Hodgson GS, Bradley TR.Properties of haematopoietic stem cells surviving 5-fluorouracil treatment: evidence for a pre-CFU-S cell?Nature. 1979;281(5730):381-382.
[12]邱蔚六.口腔颌面外科学[M].上海:上海科学技术出版社,2008: 467-487.
[13]Hong NJ, Wright FC, Gagliardi AR,et al. Examining the potential relationship between multidisciplinary cancer care and patient survival: an international literature review.J Surg Oncol. 2010;102(2):125-134.
[14]Wong RJ, Shah JP.The role of the head and neck surgeon in contemporary multidisciplinary treatment programs for advanced head and neck cancer.Curr Opin Otolaryngol Head Neck Surg. 2010;18(2):79-82.
[15]Singh SK, Clarke ID, Terasaki M,et al.Identification of a cancer stem cell in human brain tumors.Cancer Res. 2003; 63(18):5821-5828.
[16]王景涛,季平,邓诚,等.无血清培养法分选舌癌侧群细胞及其特性的鉴定[J].第三军医大学学报,2011,33(8):818-821.
[17]Li R, Wu X, Wei H,et al.Characterization of side population cells isolated from the gastric cancer cell line SGC-7901. Oncol Lett. 2013;5(3):877-883.
[18]Wang S, Xu ZY, Wang LF,et al.CD133+ cancer stem cells in lung cancer.Front Biosci (Landmark Ed). 2013;18:447-453.
[19]Shigeishi H, Biddle A, Gammon L,et al.Maintenance of stem cell self-renewal in head and neck cancers requires actions of GSK3β influenced by CD44 and RHAMM.Stem Cells. 2013; 31(10):2073-2083.
[20]Canis M, Lechner A, Mack B,et al.CD133 is a predictor of poor survival in head and neck squamous cell carcinomas. Cancer Biomark. 2012;12(2):97-105.
[21]Chen YC, Chang CJ, Hsu HS,et al. Inhibition of tumorigenicity and enhancement of radiochemosensitivity in head and neck squamous cell cancer-derived ALDH1-positive cells by knockdown of Bmi-1.Oral Oncol. 2010;46(3):158-165.
[22]Yu CC, Lo WL, Chen YW,et al. Bmi-1 Regulates Snail Expression and Promotes Metastasis Ability in Head and Neck Squamous Cancer-Derived ALDH1 Positive Cells.J Oncol. 2011;2011. pii: 609259.
[23]Wu CP, Zhou L, Xie M,et al. Identification of cancer stem-like side population cells in purified primary cultured human laryngeal squamous cell carcinoma epithelia.PLoS One. 2013; 8(6):e65750.
[24]He K, Xu T, Xu Y,et al.Cancer cells acquire a drug resistant, highly tumorigenic, cancer stem-like phenotype through modulation of the PI3K/Akt/β-catenin/CBP pathway.Int J Cancer. 2014;134(1):43-54.
[25]Teshima K, Nara M, Watanabe A,et al.Dysregulation of BMI1 and microRNA-16 collaborate to enhance an anti-apoptotic potential in the side population of refractory mantle cell lymphoma.Oncogene. 2014;33(17):2191-2203.
[26]Song J, Chang I, Chen Z,et al.Characterization of side populations in HNSCC: highly invasive, chemoresistant and abnormal Wnt signaling.PLoS One. 2010;5(7):e11456.
[27]Yanamoto S, Kawasaki G, Yamada S,et al.Isolation and characterization of cancer stem-like side population cells in human oral cancer cells.Oral Oncol. 2011;47(9):855-860.
[28]Tsai LL, Yu CC, Chang YC,et al.Markedly increased Oct4 and Nanog expression correlates with cisplatin resistance in oral squamous cell carcinoma.J Oral Pathol Med. 2011;40(8): 621-628.
[29]Grimm M, Krimmel M, Polligkeit J,et al.ABCB5 expression and cancer stem cell hypothesis in oral squamous cell carcinoma. Eur J Cancer. 2012;48(17):3186-3197.