Changes of gliacytes after spinal cord transection in Ambystoma mexicanum

doi:10.3969/j.issn.2095-4344.2013.11.014

Abstract

Abstract:

BACKGROUND: The spinal cord of Ambystoma mexicanum can regenerate after transection, and the number and distribution of gliacytes alter during regeneration. So it is important to observe the changes of gliacytes following spinal cord transection in Ambystoma mexicanum in order to further discuss the mechanism of spinal cord regeneration.
OBJECTIVE: To observe the changes of microglias, astrocytes and oligodendrocytes following spinal cord transaction in Ambystoma mexicanum.
METHODS: Adult Ambystoma mexicanum was selected and divided into spinal cord transection group and control group. Immunohistochemistry assay was performed to observe the changes in cd11b-labeled microglias, glial fibrillary acidic protein labeled astrocytes and myelin basic protein labeled oligodendrocytes in the injured region and peripheral region at 1, 3 and 10 days after spinal cord transection.
RESULTS AND CONCLUSION: After transaction, the staining results of cd11b were negative. The staining intensity results of glial fibrillary acidic protein and myelin basic protein positive cells at 3 and 10 day were lower to the control group, but showed no significant difference from the control group at 1 day after transection. Microglias were negative in Ambystoma mexicanum, indicating the marker proteins of microglia in salamander may be different from those in the mammals. The staining intensity results of glial fibrillary acidic protein and myelin basic protein positive cells at 3 and 10 days were lower to the control group, which suggested that astrocytes and oligodendrocytes hyperplasia were not seen and no glial scar formed in early stage of spinal cord injury in Ambystoma mexicanum.

Key words: tissue construction, cytology experiment in tissue construction, spinal cord transecion, microglias, astrocytes, oligodendrocytes, Ambystoma mexicanum, cd11b, glial fibrillary acidic protein, myelin basic protein, the National Natural Science Foundation of China, tissue construction photographs-containing paper

CLC Number:

R318

Li Min, Liu Jia, Zhong Yu-hua, Peng Fu-hua. Changes of gliacytes after spinal cord transection in Ambystoma mexicanum[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(11): 1993-2000.

Figures/Tables 5

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