Biomechanical changes of injured rabbit tendon-to-bone interface during repair with calcium phosphate cement-II

doi:10.3969/j.issn.2095-4344.2014.25.001

Abstract

Abstract:

BACKGROUND: Calcium phosphate cement and recombinant human bone morphogenetic protein-2 both have bone osteoinductivity. Maybe both of them could promote repair of tendon-to-bone interface damage.
OBJECTIVE: To evaluate the effect of calcium phosphate cement-II containing recombinant human bone morphogenetic protein-2 on tendon-to-bone interface healing after rotator cuff injury .
METHODS: Twenty-seven adult healthy rabbits were enrolled in the study. The tendon-to-bone interface of the bilateral shoulder joints was taken from three rabbits as normal group, and the bilateral shoulder joints of the other 24 rabbits were subjected to acute rupture of the rotator cuff and tendon-to-bone reconstructive surgery as experimental group (n=12) and control group (n=12). Rabbits in the experimental group were treated with calcium phosphate cement-II, while those in the control group treated with nothing. Specimens were collected at 2, 4, 8 postoperative weeks for biomechanical test.
RESULTS AND CONCLUSION: The maximum tensile strength of the rotator cuff in the experimental group was higher than that in the control group (P < 0.001), but lower than that in the normal group (P < 0.001). At postoperative 8 weeks, the rigidity of rotator cuff of the experiment group was higher than the control group (P < 0.001) but lower than the normal group (P < 0.001). These findings indicate that calcium phosphate cement-II can improve the maximum tensile strength and rigidity of rabbit tendon-to-bone interface in the early postoperative period, enhance tendon-to-bone interface binding force, and promote tendon-to-bone interface healing.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

全文链接：

Key words: calcium phosphates, biomechanics, bone morphogenetic proteins

CLC Number:

R318

Zhang Xi-hai, Huang Shu-hua, Li Sen, Lu Xiao-bo. Biomechanical changes of injured rabbit tendon-to-bone interface during repair with calcium phosphate cement-II[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(25): 3937-3941.

Figures/Tables 3

References

[1] Harvie P,Ostlere SJ,Teh J,et al.Genetic influences in the aetiology of tears of the rotator cuff. Sibling risk of a full-thickness tear.J Bone Joint Surgery Br. 2004;86(5): 696-700.
[2] 王永健,敖英芳.自体半腱肌腱重建兔前交叉韧带腱骨愈合和止点形成实验研究[J].中国运动医学杂志,2007,26(1):5-9.
[3] Yamagiwa H,Endo N,Tokunaga K,et al.In vivo bone-forming capacity of human bone marrow-derived stromal cells is stimulated by recombinant human bone morphogenetic protein-2.J Bone Miner Metab.2001;19(1):20-28.
[4] Rodeo SA.Biologic augmentation of rotator cuff tendon repair.J Shoulder Elbow Surg.2007;16(5 Suppl):S191-197.
[5] Zuscik MJ,Baden JF,Wu Q,et al.5-azacytidine alters TGF-beta and BMP signaling and induces maturation in articular chondrocytes.J Cell Biochem.2004;92(2):316-331.
[6] 付国建,靳安民,张力,等.rhBMP-2 对兔肩袖损伤重建术后腱骨愈合的组织学及生物力学研究[J].中国矫形外科杂志,2010, 18(10):828-831.
[7] 周宁峰,王金武,刘昌胜,等.负载 rhBMP-2的CPC活性人工骨修复骨缺损的临床应用[J].中国修复重建外志, 2009,23(3): 257-260.
[8] 郑建平,田琴,杨杰山,等.磷酸钙水泥对自体腘绳肌腱重建前交叉韧带后腱-骨愈合影响的生物力学研究[J].临床骨科杂志, 2010, 13(2):208-210.
[9] Tien YC,Chih TT,Lin JH,et al.Augmentation of tendon-bone healing by the use of calcium-phosphate cement.J Bone Joint Surg(Br).2004;86(7):1072-1076.
[10] Huangfu X,Zhao J.Tendon-bone healing enhancement using injectable tricalcium phosphate in a dog anterior cruciate ligament reconstruction model.Arthroscopy. 2007;23(5): 455-462.
[11] Urist MR.Bone: formation by autoinduction ,1965.Clin Orthop Relat Res.2002;150(395):4-10.
[12] Ma CB,Kawamura S,Deng XH,et al.Bone morphogenetic proteins signaling plays a role in tendon–to-bone healing:a study of rhBMP-2 and noggin.Am J Sports Med. 2007;35(4): 597-604.
[13] Singh K, Smucker JD, Boden SD,et al. Use of recombinant human bone morphogenetic protein-2 as an adjunct in posterolateral lumbar spine fusion: a prospective CT-scan analysis at one and two years.J Spinal Disord Tech. 2006;19: 416-423.
[14] 徐建强,周密,张树明,等.聚乳酸-乙醇酸共聚物-磷酸三钙-骨形态发生蛋白2人工骨结合肌肉移植修复骨缺损[J].中国组织工程研究与临床康复,2011,15(34):6281-6284.
[15] Jones AL,Bucholz RW,Bosse MJ,et al.Recombinant human BMP-2 and allograft compared with autogenous bone graft for reconstruction of diaphyseal tibial fractures with cortical defects A randomized,con-trolled trial.J Bone Joint Surg Am. 2006;88(7):1431-1441.
[16] Swiontkowski MF,Aro HT,Donell S,et al.Recombinant human bone morphogenetic protein-2 in open tibial fractures.a subgroup analysis of data combined from two prospective randomized studies. J Bone Joint Surg Am. 2006;88(6): 1258-1265.
[17] Znseik MJ,Baden JF,Wu Q.5-azaeytidine alters TGF-beta and BMP signaling and induces maturation in articular chondrocytes. J Cell Biochem.2004;92(2):316-331.