Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (18): 3411-3416.doi: 10.3969/j.issn.1673-8225.2012.18.039

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Research progress on vitrification and transplantation of ovarian tissue

Qin Ying, Li Mu-jun   

  1. Reproductive  Medicine Center, the First Affiliated Hospital of Guangxi Medical University, Nanning  530021, Guangxi Zhuang Autonomous Region, China
  • Received:2012-01-02 Revised:2012-02-13 Online:2012-04-29 Published:2012-04-29
  • Contact: Li Mu-jun, Doctor, Doctoral supervisor, Master’s supervisor, Chief physician, Professor, Reproductive Medicine Center, the First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China
  • About author:Qin Ying★, Studying for master’s degree, Reproductive Medicine Center, the First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China qinying834@sina. com

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Abstract:

BACKGROUND: As a rapid, simple and inexpensive freeze method, vitrification has gradually been applied to the preservation of ovarian tissues.
OBJECTIVE: To review the domestic and foreign research progress on vitrification and transplantation of ovarian tissue.
METHODS: The PubMed database and Tsinghua Tongfang database were used to retrieve the literatures about vitrification of ovarian tissue and transplantation technology of ovarian tissue from 1995 to 2011 by the first author.
RESULTS AND CONCLUSION: Vitrification is an ultrarapid cooling process that can produce a glasslike solidification of cells by extreme elevation in viscosity. Cellular injury caused by ice crystals can be avoided. However, there was not uniform standardized protocol of vitrification. The main factors that can influence the effect of vitrification are the size of ovarian tissue, the kind of cryoprotectants, equilibration times and temperatures, carrier and so on. Ovarian tissue transplantation has the clinically feasible with advances of cryobiology development and the effective cryopreservation of ovarian tissue. It has been reported that a series of successful transplantations of cryopreserved ovarian tissue that resulted in a pregnancy and live birth in the world until now. The key of successful transplantation is to reduce ischemic reperfusion injury and promote neovascularization.

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