BACKGROUND: Nano-hydroxyapatite/polyamide (nHA/PA) 66 materials are beneficial to osteoblast regeneration and new bone formation. Their bending strength and compressive strength are close to mechanical properties of normal bone tissues to meet the needs of hard tissue repair in animal experiment.
OBJECTIVE: To explore the biocompatibility of nHA/PA66 materials composite with human umbilical cord mesenchymal stem cells (hUCMSCs) after osteogenic induction.
METHODS: The hUCMSCs were isolated, cultured and osteoblastic-induced in vitro. The third-generation hUCMSCs were seeded on the nHA/PA66 materials. The growth and proliferation of hUCMSCs and cytotoxicity materials were observed.
RESULTS AND CONCLUSION: The osteoblastic-induced hUCMSCs grew well on the composite scaffolds, and proliferative activity of the cells did not change. The levels of alkaline phosphatase were gradually increased as the time of culture went on in the first 14 days of osteogenic induction. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay results showed that the cells were atoxic. Scanning electron microscopic observation: the cells showed the attached growth on the surfaces of nHA/PA66 scaffolds at 1 day; the cells grew well on the surface of nHA/PA66 scaffold and extended into the interspace of the scaffolds at 7 days. The nHA/PA66 materials can be used as cell carrier for hUCMSCs. They can satisfy the demand of bone tissue engineering due to their good biocompatibility.