Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (16): 2927-2930.doi: 10.3969/j.issn.1673-8225.2012.16.018

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Synthesis and detection of magnetic poly lactic acid-glycolic acid copolymer-bone morphogenetic protein 2 microspheres applied in tissue engineered bone  

Tian Zhi-chao1, Yang Yang2, Xiao Jun1, Qiu Jin-jun3, Zhu Yuan-li4, Li Liang-yu1   

  1. 1Department of Orthopedics, 4Department of Pathology, Tongji Hospital, Tongji Medical College of Huazhong University of Science and Technology, Wuhan  430030, Hubei Province, China; 2Department of Information Engineering, Zhengzhou Huaxin College, Zhengzhou  451100, Henan Province, China; 3School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan  430074, Hubei Province, China
  • Received:2011-09-17 Revised:2011-10-25 Online:2012-04-15 Published:2012-04-15
  • Contact: Xiao Jun, Doctor, Associate professor, Department of Orthopedics, Tongji Hospital, Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430030, Hubei Province, China xiaojun0312@ gmail.com
  • About author:Tian Zhi-chao★, Studying for master’s degree, Department of Orthopedics, Tongji Hospital, Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430030, Hubei Province, China tianzhichaoyy@ 163.com
  • Supported by:

    Research Program in Science and Technology of Wuhan, No. 200960223069*

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Abstract:

BACKGROUND: Poor porosity and the lack of the ability to provide bioactive factors for seed cells in an appropriate way of tissue-engineered bone scaffolds may lead to weak adhesion and induction of the scaffolds to cells, and low osteogenic efficiency.
OBJECTIVE: To synthesis a new kind of magnetic poly lactic acid-glycolic acid copolymer-bone morphogenetic protein 2 (PLGA-BMP2) microspheres that can be used in tissue engineered bone scaffolds and detect their various performance.
METHODS: The PLGA-BMP2 microspheres were prepared with double emulsion method.
RESULTS AND CONCLUSION: The scanning electron microscopy images showed that the microspheres were positive round with the particle size of 10-100 µm. The laser scanning confocal microscope images showed that human BMP2 evenly distributed on the microspheres. The microspheres had superparamagnetism detected by vibrating sample magnetometer. The microspheres had the sustained-release effect and their drug loading was (1.00±0.18) μg/g. The drug loading was (0.98±0.20) μg/g after the microspheres were treated with 45 ℃. The magnetic PLGA-BMP2 microspheres have a range of good features and lay a foundation for development of newly magnetic tissue engineered bone.

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