Abstract:

BACKGROUND: Several literatures have questioned that with the increase of nano hydroxyapatite sol concentration, the capacity of killing tumor cells becomes stronger.
OBJECTIVE: To investigate the killing effect of nano hydroxyapatite sol with different concentrations on Hela cells.
METHODS: Nano hydroxyapatite sol with the concentrations of 5, 10, 20, 40, 80, 160, 320 and 640 μg/mL were synthesized by the chemical precipitation method and added into the Hela cells. The Hela cells added with 5 and 10 mg/L taxol served as controls.
RESULTS AND CONCLUSION: ①Nano hydroxyapatite sol with different concentrations had killing effect on Hela cells; as the concentration of nano hydroxyapatite sol increased; the killing effect was gradually improved. But their inhibited effect was not better than that of taxol. ②The killing effect of nano hydroxyapatite sol and the taxol on Hela cells began decreased at day 2, but it increased in the following day. The inhibited effect at day 3 was more obvious than that at day 1, and nano hydroxyapatite sol with various concentrations were all showed this tendency. ③A certain inhibited effect of nano hydroxyapatite sol on Hela cells was found. The proliferation of Hela cells was not significant after given taxol, while Hela cells were found proliferation in various degrees after given nano hydroxyapatite sol.