BACKGROUND: Nanometer technique is one of the effective ways to improve chemotherapy sensitivity of tumor cells by refining dosage form and target action of chemotherapy.
OBJECTIVE: To investigate the depression effect of carbon coated iron nanoparticles carrying diamminedichloroplatinum (CCINs-DDP) on Hep-2 laryngocarcinoma cells and expression of caspase 3 and survivin mRNA.
METHODS: Hep-2 laryngocarcinoma cells were intervened by iron-carbon nanoparticles and (or) normal saline dispersion of cisplatin. Cells interfered with normal saline were selected as controls.
RESULTS AND CONCLUSION: Methylthiazolyldiphenyl-tetrazolium bromide assay showed that cisplatin could inhibit cell growth of Hep-2 cells, and combined with the iron-carbon nanoparticles, the inhibitory effect was stronger. Cells were not adherent, appeared with slow proliferation and apoptosis. Reverse transcription-PCR results showed that caspase 3 mRNA level of the Hep-2 cells was significantly increased at co-cultured 5 days after treated with iron-carbon nanoparticles combined with cisplatin and simple treated with the cisplatin, but the expression of survivin mRNA was significantly decreased, which was more obviously after treated with iron-carbon nanoparticles combined with cisplatin, but simple application of iron-carbon nanoparticles had no effect on Hep-2 cells.
It is indicated that CCINs-DDP can improve the chemotherapy sensitivity of Hep-2 laryngocarcinoma cells on the DDP and also improve therapeutic efficacy of chemotherapeutics.