Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (12): 2129-2132.doi: 10.3969/j.issn.1673-8225.2012.12.009

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Nano-hydroxyapatite/bone morphogenetic protein-2 culture with bone marrow stem cells in vitro*☆

Dai Ping1, Zhang Hong2, Chen Xing-lai1, Yuan Jian-hui3   

  1. 1Department of Orthopedics, Fuyong People's Hospital of Baoan District, Shenzhen  518103, Guangdong Province, China;    2Shenzhen Health Bureau New Hospitals Preparatory Office, Shenzhen  518020, Guangdong Province, China;    3Shenzhen Center for Disease Control and Prevention, Shenzhen  518000, Guangdong Province, China
  • Received:2011-08-22 Revised:2011-10-22 Online:2012-03-18 Published:2012-03-18
  • About author:Dai Ping☆, Studying for doctorate, Associate chief physician, Department of Orthopedics, Fuyong People's Hospital of Baoan District, Shenzhen 518103, Guangdong Province, China dpqiangtongding@ yahoo.com.cn

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Abstract:

BACKGROUND: Whether a combination of the nano-hydroxyapatite (nHA) scaffolds with bone morphogenetic protein-2 (BMP-2) is superimposed to promote or inhibit the bone marrow stromal stem cells, as well as its biocompatibility, remain unclear.
OBJECTIVE: To observe the biocompatibility and bone formation activity of nHA/BMP-2 co-cultured with rabbit bone marrow stem cells in vitro.
METHODS: Bone marrow stem cells obtained from rabbits were cultured and proliferated in DMEM medium in vitro. The cells at the third passage were inoculated into four mediums and according divided into four groups: control group, BMP-2 group, nHa group, and nHa/BMP-2 group.
RESULTS AND CONCLUSION: The cell diplo-proliferation time of control group and nHa group was longer than that in BMP-2 group and nHa/BMP-2 group. The cells proliferative activity calculated by MTT assay and bone formation activity based on the alkaline phosphatase method demonstrated statistical significant difference in BMP-2 group and nHa/BMP-2 group compared with control and nHa group (P < 0.01). Experimental findings indicate that, the tissue formation of nHA/BMP-2 not only has good biocompatibility with Bone marrow stem cells, but also could bring into full play the effect of BMP-2 for promoting the proliferation, differentiation, and bone formation activity of bone marrow stem cells.

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