Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (6): 963-967.doi: 10.3969/j.issn.1673-8225.2012.06.003

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Isolation and culture of human bone marrow mesenchymal stem cells and sweat gland cells in vitro

Ai Li1, Weng Li-xin1, Sun Tong-zhu2, Sun Hong3   

  1. Department of Pathology, Affiliated Hospital of Inner Mongolia Medical College, Hohhot  010059, Inner Mongolia Autonomous Region, China; 2Laboratory of Wound Repair, First Department of Clinical Medicine, General Hospital of Chinese PLA, Beijing  100853, China; 3Office of Basic Medical Education and Research, Inner Mongolia Medical College, Hohhot  010059, Inner Mongolia Autonomous Region, China
  • Received:2011-12-03 Revised:2011-12-29 Online:2012-02-05 Published:2012-02-05
  • Contact: Weng Li-xin, Master, Associate professor, Department of Pathology, Affiliated Hospital of Inner Mongolia Medical College, Hohhot 010059, Inner Mongolia Autonomous Region, China wenglixin2007@yahoo.cn
  • About author:Ai Li★, Master, Attending physician, Department of Pathology, Affiliated Hospital of Inner Mongolia Medical College, Hohhot 010059, Inner Mongolia Autonomous Region, China
  • Supported by:

    Scientific Research Project, Department of Education of Inner Mongolia Autonomous Region, No. NJZY07093*

Abstract:

BACKGROUND: In vitro research on the culture and identification of bone marrow mesenchymal stem cells (BMSCs) and sweat gland cells can provide the foundation for the feasibility of BMSCs regenerating sweat gland.
OBJECTIVE: To study the effective method on isolation and culture of sweat gland cells and human BMSCs in vitro.
METHODS: The BMSCs were isolated and cultured from adult bone marrow by adherent method, and then expanded and identified in vitro. Sweat gland cells were separated from human full thickness burnless skin using collagenase digestion method, and expanded and identified.
RESULTS AND CONCLUSION: The separated and cultured BMSCs showed spindle-shaped and strong refraction under inverted microscope. Immunocytochemistry staining shows that CD29, CD44, CD105 were positive in BMSCs, surface markers CD34 and CD45 of hematopoietic stem cells were negative. Sweat gland cells showed flat polygonal, and surface markers cytokeratin (CK) 7, CK8, CK18, CK19 and carcinoembryonic antigen were positive in sweat gland cells. It is feasible to separate and culture BMSCs with adherent method and to isolate and culture sweat gland cells with collagenase digestion method.

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