Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (47): 8762-8764.doi: 10.3969/j.issn.1673-8225.2011.47.006

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Expression of integrin alpha 5 beta 1 during the adhesion of osteoblasts to bio-derived materials

Qi Jie1, Zhang Shu-jiang2, Liu Zong-zhi1   

  1. 1Department of Orthopeadic Surgery, Shaanxi Provincial People’s Hospital, Xi’an  710068, Shaanxi Province, China
    2Orthopedics Laboratory, First Affiliated Hospital of Gangzhou Medical College, Guangzhou  510120, Guangdong Province, China
  • Received:2011-07-14 Revised:2011-09-01 Online:2011-11-19 Published:2011-11-19
  • About author:Qi Jie☆, Doctor, Associate chief physician, Department of Orthopeadic Surgery, Shaanxi Provincial People’s Hospital, Xi’an 710068, Shaanxi Province, China qj_dentist@163.com
  • Supported by:

    the Science and Technology Research and Development Plan of Shaanxi Province, No. 2008K14-07*; the Natural Science Foundation of Shaanxi Province, No. 2009JM4017*

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Abstract:

BACKGROUND: Integrin plays an important role in the cell adhesion process.
OBJECTIVE: To explore the expressions and function of integrinα5β1 during the adhesion of osteoblasts to bio-derived materials.
METHODS: Fetal osteoblasts as seed cells were used to construct tissue engineered bone and periosteum following incubation on the bio-derived materials, which were cultured for 2, 4, 6, 8, 10 days in vitro. Osteoblasts cultured alone served as controls.
RESULTS AND CONCLUSION: Integrinα5 did not express at the early-stage. Integrinβ1 kept expressing in all groups, and expressed higher on bio-derived bone than on tissue-engineered periosteum, especially at the 2nd and 6th days. Integrinβ1 expressed stably in the control group. The bio-derived materials promote osteoblasts adhesion but fibronectin is not essential to the adhesion of osteoblasts.

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