Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (47): 8757-8761.doi: 10.3969/j.issn.1673-8225.2011.47.005

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Preparation of surface-patterned biodegradable poly(organophosphazene) and its affinity to osteoblasts

Cai Qing1, Qi Bing1, Duan Shun1, Mao Ji-fu1, Shen Hong2, Yang Fei2, Yang Xiao-ping1, Wang Shen-guo2   

  1. 1Key Laboratory of Carbon Fiber and Functional Polymers, Ministry of Education, Beijing University of Chemical Technology, Beijing  100029, China
    2Center for Molecular Science, Institute of Chemistry, Chinese Academy of Science, Beijing  100190, China
  • Received:2011-04-05 Revised:2011-07-05 Online:2011-11-19 Published:2011-11-19
  • Contact: Shen Hong, Ph.D., Assistant researcher, Center for Molecular Science, Institute of Chemistry, Chinese Academy of Science, Beijing 100190, China shenhong516@ iccas.ac.cn
  • About author:Cai Qing☆, Ph.D., Associate professor, Key Laboratory of Carbon Fiber and Functional Polymers, Ministry of Education, Beijing University of Chemical Technology, Beijing 100029, China caiqing@ mail.buct.edu.cn
  • Supported by:

    the National Natural Science Foundation of China, No. 50873012*, 51073016*

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Abstract:

BACKGROUND: It has been identified that surface topographical structures in nano- or micro-scale can significantly affect the adhesion and proliferation of cells on artificial matrixes, and promote cell behaviors.   
OBJECTIVE: To investigate the effect of preparation parameters on the surface characteristics of poly(glycine ethyl ester-co- alaine ethyl ester)phosphazene (PGAP) films, and the osteoblasts behaviors on PGAP films.  
METHODS: PGAP was dissolved in chloroform and polymeric solutions with different concentrations were obtained. Then the solutions were cast onto PTFE plates under relative humidity (RH) of 20% or 80% at 25 ℃. After 2 hours of solvent evaporation, the samples were vacuum-dried at 25 ℃ to constant weight. The resulted films were characterized with scanning electron microscope, atomic force microscope, and X-ray diffraction and water contact angle measurement to determine their surface morphology, composition and hydrophilicity. Mouse osteoblast-like cells OCT-1 were seeded onto the PGAP films, and the effect of surface features of PGAP films on cell behaviors were evaluated via determination of cell adhesion efficiency, proliferation and morphology.  
RESULTS AND CONCLUSION: The surface morphology and composition of solvent-cast PGAP films depended closely on the solution concentration and environmental RH. In comparison with smooth PGAP films prepared under low RH, surface-patterned PGAP films were obtained under high RH which contributed high surface roughness. The porous structure turned more regular as the solution concentration increasing, a kind of honeycomb structure was observed at the concentration of 120 g/L. However, the surface pattern was blurred when the solution concentration was further increased to 160 g/L, owing to the increased viscosity and the slowed solvent evaporation. Accumulation of polar phosphorous and nitrogen atoms on film surface has been found in the cases of PGAP films prepared under RH of 80%, which resulted in high protein absorption ability in combination with the increased surface roughness. These features facilitated the adhesion, spread and proliferation of mouse osteoblast-like cells OCT-1. All the results suggested that parameters like RH and solution concentration could be an easy way to alter the surface properties of PGAP films and thus could be used in the study of cell-matrix interaction.

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