Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (42): 7822-7826.doi: 10.3969/j.issn.1673-8225.2011.42.008

Previous Articles     Next Articles

Cytocompatibility of stem cells-derived osteoblasts with porous calcium carbonate ceramics

Wei Ren-xiong, Zhang Yi, Tan Jin-hai, Cai Lin, Jin Wei   

  1. Department of Orthopedics, Zhongnan Hospital of Wuhan University, Wuhan  430071, Hubei Province, China
  • Received:2011-07-04 Revised:2011-08-12 Online:2011-10-15 Published:2011-10-15
  • Contact: Tan Jin-hai, Doctor, Chief physician, Professor, Master’s supervisor, Department of Orthopedics, Zhongnan Hospital of Wuhan University, Wuhan 430071, Hubei Province, China guke3224@ yahoo.com.cn
  • About author:Wei Ren-xiong☆, Doctor, Attending physician, Department of Orthopedics, Zhongnan Hospital of Wuhan University, Wuhan 430071, Hubei Province, China Feiyi030@163.com
  • Supported by:

    the Key Grant of Natural Science Foundation of Hubei Province, No. 2009CDA058*

PDF

245

Abstract:

BACKGROUND: Foreign studies have confirmed that calcium carbonate ceramics as bone substitute materials has a cytoskeletal role.
OBJECTIVE: To study the cytocompatibility of osteoblasts with porous calcium carbonate ceramics as bone tissue engineering scaffold.
METHODS: Bone marrow stromal cells (BMSCs) obtained from SD rat bone marrow were in vitro induced and proliferated. After their osteoblast phenotypes were verified, osteoblasts which cultured from BMSCs were seeded onto prepared porous calcium carbonate ceramics (PCCC group) and common hydroxyapatite ceramics (HA group).
RESULTS AND CONCLUSION: Immunological staining results for calcium nodules, Ⅰ collagen and alkaline phosphatase were positive. Adherent osteoblasts were found in the two groups. However, the adherent ability, proliferative ability and osteogenic activity in the PCCC group were stronger than those in the HA group. It indicates that the porous calcium carbonate ceramics has good cytocompatibility with BMSCs-derived osteoblasts.

CLC Number: