Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (40): 7545-7548.doi: 10.3969/j.issn.1673-8225.2011.40.031

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Transition of Cleaved Notch1 protein and gene expression changes in myeloblastic leukemia cells stimulated with Notch ligands

Fu Lu, Wu Ai-min, Li Jie, Wu Yue, Zhang Yun-ping, Xin Xiao-min   

  1. Department of Laboratory Diagnosis, First Affiliated Hospital of Harbin Medical University, Harbin 150001, Heilongjiang Province, China
  • Received:2011-04-06 Revised:2011-05-17 Online:2011-10-01 Published:2011-10-01
  • About author:Fu Lu☆, Doctor, Attending physician, Department of Laboratory Diagnosis, First Affiliated Hospital of Harbin Medical University, Harbin 150001, Heilongjiang Province, China Fulu_2002@163.com
  • Supported by:

    the Overseas Scholar Foundation of Education Department of Heilongjiang Province, No. 1155h011*

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Abstract:

BACKGROUND: Notch activation by ligand stimulation regulates the growth of acute myeloid leukemia cells. However, the molecular mechanisms underlying this have not been fully elucidated.
OBJECTIVE: To observe the effect of cleavage of Notch1 protein and gene expression in the leukemia cell lines stimulated with Notch ligands.
METHODS: Two acute myeloid leukemia cell lines, THP-1 and TMD7, and three Notch ligands, Jagged1, Dll1 and Dll4, were used. The effects of the ligands on cell growth, cleavage of Notch1, and the expression of various genes were examined by cell culture, immunoblotting, and quantitative RT-PCR, respectively.
RESULTS AND CONCLUSION: Ligand stimulation suppressed the growth of the THP-1 cells but promoted that of the TMD7 cells. Immunoblots showed two Cleaved Notch1 bands, which became intense with different peak time from the start of stimulation. Ligand stimulation changed the expression levels of various genes related to cell proliferation. Some genes changed in opposite directions in the two cell lines. The three ligands had similar effects on gene expression.

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