Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (40): 7446-7449.doi: 10.3969/j.issn.1673-8225.2011.40.009

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Osteogenic differentiation of mesenchymal stem cells from human cord blood in vitro

Wang Hao1,2, Wei Dong-xing2   

  1. 1Department of Orthopedic Surgery, the Fifth Clinical Hospital of Liaoning Medical University, Jinzhou  121000, Liaoning Province, China
    2Department of Orthopedic Surgery, Jinzhou Central Hospital, Jinzhou  121000, Liaoning Province, China
  • Received:2011-04-07 Revised:2011-05-30 Online:2011-10-01 Published:2011-10-01
  • About author:Wang Hao★, Studying for master’s degree, Associate chief physician, Department of Orthopedic Surgery, the Fifth Clinical Hospital of Liaoning Medical University, Jinzhou 121000, Liaoning Province, China; Department of Orthopedic Surgery, Jinzhou Central Hospital, Jinzhou 121000, Liaoning Province, China yunshengma@126.com

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Abstract:

BACKGROUND: Negative screening for immune cells can remove the effect of hematopoietic stem cells on the growth of mesenchymal stem cells, with rapid access to obtain cell populations to express mesenchymal stem cells.
OBJECTIVE: To study the osteogenic ability of mesenchymal stem cells from human cord blood.
METHODS: The mesenchymal stem cells were isolated by immunomagnetic beads from cord blood. P2 cells were to study the ability of osteogenesis.
RESULTS AND CONCLUSION: The human cord blood mesenchymal stem cells presented spindle shape and reached confluence within 3 weeks. The phenotypes were CD44+, CD29+, CD166+, CD34-. In osteogenic experiment, the cells’ alkaline phosphatase expression was positive, and hydroxyapatite nodules were detected by Von-Kossa staining. The human cord blood mesenchymal stem cells can differentiate into osteoblast-like cells in vitro.

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