Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (38): 7127-7131.doi: 10.3969/j.issn.1673-8225.2011.38.022

Previous Articles     Next Articles

Mechanical properties of hyaluronic acid modifying chitosan/collagen/nano-hydroxyapatite composite scaffold and its effect on osteoblast proliferation

Liu Lu1, 2, Li Rui-xin1, Zhang Li2, Guo Yong1, Chen Xue-zhong1, Wang Liang1, Dong Li-zhi1, Zhang Xi-zheng1   

  1. 1 Institute of Medical Equipment of the Academy of Military Medical Sciences of Chinese PLA, Tianjin  300161, China
    2Medical College of Chinese People’s Armed Police Forces, Tianjin  300162, China
  • Received:2011-03-19 Revised:2011-03-28 Online:2011-09-17 Published:2011-09-17
  • Contact: Zhang Xi-zheng, Doctor, Researcher, Institute of Medical Equipment of the Academy of Military Medical Sciences of Chinese PLA, Tianjin 300161, China z56787@sohu.com
  • About author:Liu Lu☆, Studying for doctorate, Institute of Medical Equipment of the Academy of Military Medical Sciences of Chinese PLA, Tianjin 300161, China; Medical College of Chinese People’s Armed Police Forces, Tianjin 300162, China netingcat@163.com
  • Supported by:

    the National Natural Science Foundation of China, No. 10832012*

PDF

444

Abstract:

BACKGROUND: Seed cell exerting its function is required to depend on the extracellular matrix in tissue engineering, so that biocompatible material is important to be selected.
OBJECTIVE: To prepare a novel composite scaffolds of chitosan/collagen/nano-hydroxyapatite (HA-CS/Col/nHAP) and to optimize the technology of tissue engineered-stents according to the circumstances of cell adhesion.
METHODS: Chitosan was modified by hyaluronate acid. The structure was observed by differential scanning calorimetry and the Fourier transformed infrared spectroscopy. Three composites of HA-CS/Col/nHAP according to different ratio of chitosan and collagen solution (1: 2; 1: 1 and 2: 1) were prepared. The composite scaffolds were co-cultured with osteoblast MC3T3-E1, and the proliferation and cell growth curve were measured by CCK-8 method.
RESULTS AND CONCLUSION: Hyaluronic acid and chitosan were crosslinked with amide linkage. Pore size was on the range from 50 μm to 250 μm. Porosity was increased with increased collagen level and elastic modulus, but density was reduced. Increased collagen content was beneficial for cell adhesion and proliferation on stent in the primary phase of cell co-culture. However, from day 10, no significant difference was determined among three samples. At the beginning of cell culture, cells adhered to the airspace insides the composite scaffolds. In the following days, cells grew in a colony manner, and cell-cell junction could be easily observed. These indicate that HA-CS/Col /nHAP composite scaffolds can improve the adhesion and proliferation of osteoblast. The ratio of chitosan to collagen volume at 1: 1 was optimal.

CLC Number: