Abstract:

BACKGROUND: Cell transplantation is a promising strategy for treatment of spinal cord injury. But the current experimental evidence about cerebrospinal fluid (CSF) effect on the cells is lack.
OBJECTIVE: To study the CSF effects on the in vitro culture of bone marrow mesenchymal stem cells (BMSCs).
METHODS: BMSCs were isolated and purified by density gradient centrifugation and adherent culture, and BMSCs were passaged in vitro. The third and fourth passage cells were cultured in CSD to observe cells growth conditions, and analyze some of its phenotypic characteristics. The fifth passage cells were cultured in L-DMEM containing fetal calf serum and CSF.
RESULTS AND CONCLUSION: We had successfully and effectively isolated and purified BMSCs by density gradient centrifugation with adherent culture. The results of the identification were positive of CD90, CD106, CD71, CD29, while CD45 was negative. Cells cultured in CSF still had still morphology of BMSCs. But few cells were neural-like cells. The results of the identification were positive of CD90, CD106, CD71, CD29, while CD45 was negative. Neuron-specific enolase was weakly positive. Cells had a similar S-shaped growth curve, and the growth curves were similar. BMSCs can still proliferate in CSF, thus it indicate that these cells possess high adaptability to their growth environment.