Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (32): 6041-6044.doi: 10.3969/j.issn.1673-8225.2011.32.034

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Effects of AMLl-ETO fusion gene on the expression of C/EBPα

Zhuang Wen-yue1, 2, 3, Chen Zi-xing1, 2, Zhao Yun1, 2, Cen Jian-nong2, Shen Hong-jie2, Qi Xiao-fei2, Li Bing-zong4   

  1. 1Medical College of Soochow University, Suzhou  215000, Jiangsu Province, China
    2The First Affiliated Hospital of Soochow University, Jiangsu Institute of Hematology, Suzhou  215000, Jiangsu Province, China
    3Medical Ecsomatics College of Beihua University, Jilin  132013, Jilin Province, China
    4Department of Hematology, the Second Affiliated Hospital of Soochow University, Suzhou  215004, Jiangsu Province, China
  • Received:2011-01-13 Revised:2011-03-18 Online:2011-08-06 Published:2011-08-06
  • Contact: Chen Zi-xing, Doctoral supervisor, Medical College of Soochow University, Suzhou 215000, Jiangsu Province, China; The First Affiliated Hospital of Soochow University, Jiangsu Institute of Hematology, Suzhou 215000, Jiangsu Province, China
  • About author:Zhuang Wen-yue☆, Studying for doctorate, Medical College of Soochow University, Suzhou 215000, Jiangsu Province, China; The First Affiliated Hospital of Soochow University, Jiangsu Institute of Hematology, Suzhou 215000, Jiangsu Province, China; Medical Ecsomatics College of Beihua University, Jilin 132013, Jilin Province, China Lzyzwy1234@yahoo.com.cn

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Abstract:

BACKGROUND: Recent studies have shown that function of C/EBPα gene can be inhibited by AML1-ETO resulting in differentiation block.
OBJECTIVE: To investigate the effects of AML1-ETO fusion gene on transcription factor C/EBPα which is crucial for the differentiation of granulocytes, and to explore its role in leukemogenesis.
METHODS: The expression of cell differentiation antigen including CD11b and CD14 was detected by flow cytometry. C/EBPα gene expression was assessed by quantitative PCR, and C/EBPα protein expression was tested by Western blot. The chromatin immunoprecipitation (ChIP)-based PCR was used to investigate the direct interaction between the AML1-ETO and C/EBPα promoter in AML1-ETO positive leukemia cell line.
RESULTS AND CONCLUSION: The expression of cell differentiation antigen CD11b and CD14 significantly decreased in transfected cells. C/EBPα expression in both mRNA and protein levels decreased in U937 cells transfected with pcDNA3.1-AML1-ETO. The enriched regions in transfected cells were located within C/EBPα promoter. Results indicated that C/EBPα is the direct target gene of AML1-ETO. AML1-ETO can down-regulate the expression of C/EBPα.

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