Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (32): 6003-6006.doi: 10.3969/j.issn.1673-8225.2011.32.026

Previous Articles     Next Articles

Dynamic fluorescence in situ hybridization monitoring for chromosomal changes in patients with leukemia after chemotherapy and stem cell transplantation

Zhang Zheng-hao, Qu Jian-hua, Chen-shuang, Liu Yang, Jiang Ming, Duan Xian-lin, Yuan Hai-long, Wang Lei   

  1. Second Department of Hematology, First Affiliated Hospital of Xinjiang Medical University, Urumqi  830054, Xinjiang Uygur Autonomous Region, China
  • Received:2011-01-05 Revised:2011-02-09 Online:2011-08-06 Published:2011-08-06
  • Contact: Qu Jian-hua, Doctor, Chief physician, Second Department of Hematology, First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China jianhuaqu704@yahoo.com.cn
  • About author:Zhang Zheng-hao★, Studying for master’s degree, Physician, Second Department of Hematology, First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China Zhangzhenghao123@sohu.com

PDF

322

Abstract:

BACKGROUND: Fluorescence in situ hybridization (FISH) is widely used in the detection of abnormal genes in leukemia, but the application of this technique for comparing the efficacy of chemotherapy and transplantation has not been coverage a lot.
OBJECTIVE: To evaluate the monitoring results of FISH in patients with leukemia after chemotherapy and hematopoietic stem cell transplantation.
METHODS: A total of 85 specimens from 44 leukemia patients after chemotherapy or allogeneic stem cell transplantation were studied by FISH and conventional cytogenetic analysis. We divided the results into four groups: pretreatment group, chemotherapy group, special drug group (all-trans retinoic acid and imatinib mesylate) and hematopoietic stem cell transplantation group (transplantation group).
RESULTS AND CONCLUSION: FISH in the diagnosis and monitoring of leukemia minimal residual diseases was more accurate than conventional cytogenetic analysis; The result of FISH showed that the percentage of positive cells in the special drug and transplantation groups was lower than that in the pretreatment and chemotherapy groups (P  < 0.01), but there was no difference between the special drug and transplantation groups (P  > 0.05). The incidence of acute graft-versus-host disease had no correlation with the results of FISH at 1 month after transplantation (P  > 0.05). The FISH results at 1 month, 1-6 months, over 6 months after transplantation had no difference in statistics (P > 0.05). FISH can detect of chromosomal changes for patients after transplantation accurately and effectively, and it can be used to evaluate the trends of disease progression in patients.

CLC Number: