Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (21): 3815-3818.doi: 10.3969/j.issn.1673-8225.2010.21.004

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Recombinant adenovirus-mediated bone morphogenetic protein 9 versus bone morphogenetic protein 2 combined with nano-hydroxyapatite/polyamide composites in repair of radial defects

Lu Xiao-juan1, Zhao Ying-ze1, Wen Wei1, Zhang Ting1, Chen Heng-jun2, Zhou Wei3, Feng Tao3, Kang Quan4   

  1. 1 Key Laboratory of Clinical Laboratory, Ministry of Education, Chongqing Medical University, Chongqing  400014, China; 2 Department of Dermatology, First Affiliated Hospital of Chongqing Medical University, Chongqing  400014, China; 3 Molecular Medicine and Cancer Research Centre, Chongqing Medical University, Chongqing  400014, China; 4 Department of Pediatric Surgery, Children’s Hospital of Chongqing Medical University, Chongqing  400016, China
  • Online:2010-05-21 Published:2010-05-21
  • Contact: Kang Quan, Doctor, Associate professor, Department of Pediatric Surgery, Children’s Hospital of Chongqing Medical University, Chongqing 400016, China
  • About author:Lu Xiao-juan★, Studying for master’s degree, Key Laboratory of Clinical Laboratory, Ministry of Education, Chongqing Medical University, Chongqing 400014, China piaopiaogangan@163.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30600625/C1607*　

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Abstract:

BACKGROUND: Compared with the traditional method, this paper applies a new method of recombinant adenovirus-mediated gene expression with the advantages of high efficiency, convenience and safety, which can serve as powerful means for gene therapy.
OBJECTIVE: To compare the effect of adenovirus-mediated human bone morphogenetic protein 9 (Adv-hBMP-9) and adenovirus-mediated human bone morphogenetic protein 2 (Adv-hBMP-2) nano-hydroxyapatite / polyamide complex on reconstruction of bone defects.
METHODS: A total of 36 New Zealand rabbits were randomly divided into 3 groups and prepared for 13-mm bone defect models at the bilateral middle radius. Adv-hBMP9 + nano-hydroxyapatite / polyamide bone, Adv-hBMP-2 + nano-hydroxyapatite / polyamide bone, and Adv-GFP + hydroxyapatite / polyamide bone were implanted into the defects in each group. General observation, X-ray photographs and histological sections were carried out separately.
RESULTS AND CONCLUSION: Bone defects were completely repaired in the BMP-9 group; partly repaired in the BMP-2 group; however, reparation of bone defects was obviously poor in the GFP control group. The results revealed that effect of recombinant Adv-hBMP-9 is stronger than Adv-hBMP-2 on repairing osteogenesis after radial defects.

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