Abstract:

BACKGROUND: In vivo bone tissue engineering is to implant biological materials with bone induction activity in the bone or non-bone region. The body served as bioreactor to construct tissue engineered bone to repair bone defects. Bone transplant with good biocompatibility and mechanical function developed a new therapeutic pathway for bone transplantation. However, the source of related stem cells remains unclear.
OBJECTIVE: To investigate whether bone marrow mesenchymal stem cells (BMSCs) participate in forming ectopic bone of biomaterial.
METHODS: BMSCs were separated and purified from male beagle dogs. BMSCs at passage 3 were transplanted into the female beagle dogs to establish models of allogeneic bone marrow transplantation. Simultaneously, biphasic calcium phosphate ceramic was implanted in erector spinae of receptor dog. Following 6 weeks, bone sample was harvested. Fluorescence in situ hybridization was utilized to trace bone transplant Y chromosome.
RESULTS AND CONCLUSION: Fluorescence in situ hybridization results demonstrated that chromosome Y had fluorescent signal expression in bone constructs, which suggested that BMSCs can be collected in the ectopic biomaterial region by blood circulation, and formed bone tissue. Results indicated that BMSCs participate in inducing ectopic bone formation, as a source of inducing ectopic bone formation.