Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (15): 2661-2684.doi: 10.3969/j.issn.1673-8225.2011.15.001

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Effect of different-intensity static magnetic field on the reorganization of osteoblast cytoskeleton

Wang Sheng-guo1, Zhou Li2, Chen Yang-xi2   

  1. 1Department of Stomatology, the Second Affiliated Hospital of Chongqing Medical University, Chongqing  400010, China
    2Department of Orthodontics, West China College of Stomatology, Sichuan University, Chengdu  610041, Sichuan Province, China
  • Received:2010-11-08 Revised:2010-12-20 Online:2011-04-09 Published:2013-11-06
  • About author:Wang Sheng-guo☆, Doctor, Attending physician, Department of Stomatology, the Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China wangshengguo76@ 163.com

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Abstract:

BACKGROUND: Mechanisms addressing effects of static magnetic field on osteoblast proliferation, cytokine secretion and alkaline phosphatase (ALP) activity remain poorly understood.
OBJECTIVE: To evaluate the effects of static magnetic field loading the cytoskeleton reorganization and cytoskeletal protein expression.
METHODS: The osteoblasts were harvested from skull of SD rats within 24 hours after born and cultured. Static magnetic fields with 0, 8, 50 and 160 mT were added on osteoblasts. The BODYPY-Phaloidi was used to stain cytoskeleton, and fluorescence intensity was measured by laser scanning confocal microscope and image J at 24, 48 and 72 hours after loading.
RESULTS AND CONCLUSION: At 24, 48 and 72 hours after culturing under 8, 50 and 160 mT static magnetic fields, the cytoskeleton fluorescence concentrated to the nucleus and the fluorescence intensity up-regulated (P < 0.05), especially strongest in the 50 mT static magnetic field. The static magnetic field can result in the reorganization of the osteoblast cytoskeleton.  

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