Chinese Journal of Tissue Engineering Research ›› 2013, Vol. 17 ›› Issue (44): 7693-7697.doi: 10.3969/j.issn.2095-4344.2013.44.006

Previous Articles     Next Articles

Dynamic changes of Th1/Th2 cytokines in acute rejection after renaltransplantation

Huang Xuan, Re Yi-han, Liu Jian, Sun Yan   

  1. Department of Nephrology, the First Affiliated Hospital of Xinjiang Medical University, Key Construction Disciplines of Internal Medicine of Xinjiang Institute of Organ Transplantation, Urumqi 830054, Xinjiang Uygur Autonomous Region, China
  • Online:2013-10-29 Published:2013-10-31
  • Contact: Liu Jian, Department of Nephrology, the First Affiliated Hospital of Xinjiang Medical University, Key Construction Disciplines of Internal Medicine of Xinjiang Institute of Organ Transplantation, Urumqi 830054, Xinjiang Uygur Autonomous Region, China jianliu@medmail.com.cn
  • About author:Huang Xuan, Department of Nephrology, the First Affiliated Hospital of Xinjiang Medical University, Key Construction Disciplines of Internal Medicine of Xinjiang Institute of Organ Transplantation, Urumqi 830054, Xinjiang Uygur Autonomous Region, China

PDF

410

Abstract:

BACKGROUND: The changes of single cytokine before and after transplantation has no clinical significance in the diagnosis of acute rejection, but the joint monitoring of cytokines is better to determine the immune status of transplanted patients.

OBJECTIVE: To observe the dynamics changes of Th1/Th2 cytokines before and after transplantation in order to investigate the clinical significance in the diagnosis of acute rejection.

METHODS: It was a prospective cohort study. The Th1 and Th2 cytokine levels in the rejection group and non-rejection group were dynamically monitored with double-antibody sandwich enzyme-linked immunosorbent assay before, and 3, 7 and 14 days after transplantation.  

RESULTS AND CONCLUSION: Before renal transplantation, the Th1 (γ-interferon and interleukin-12) cytokine levels in the rejection group were significantly higher than those in the non-rejection group (P < 0.05); the Th2 (interleukin-4 and interleukin-10) cytokine levels in the rejection group were significantly lower than those in the non-rejection group (P < 0.05). After renal transplantation, there were no significant changes in Th1 and Th2 cytokines levels of the non-rejection group at different time points; the Th1 cytokine levels in the rejection group were increased rapidly at different time points, and reached a peak before acute rejection, and the Th1 cytokine levels at different time points in the rejection group weresignificantly higher than those in the non-rejection group；the Th2 cytokines were increased gradually in therejection group, and reached a peak before acute rejection, and the Th2 cytokine levels atdifferent time points in the rejection group were still lower than those in the non-rejection group. The results showed that the Th1/Th2 cytokines in the non-rejection group were kept stably before and after renal transplantation, but not in the rejection group, in which, the Th1 cytokines were increased rapidly before acute rejection and the Th2 cytokines were increased gradually.

Key words: kidney transplantation, interferon-gamma, interleukin-4, interleukin-10, interleukin-12

CLC Number: