Expression levels of Foxp3 and indoleamine 2,3-dioxygenase in mouse corneal allograft rejection

doi:10.3969/j.issn.2095-4344.0857

Abstract

Abstract:

BACKGROUND: Immunological rejection reaction is the main cause of corneal allograft failure and its pathogenesis is still unclear. Foxp3 and indoleamine 2,3-dioxygenase (IDO) are reported to be associated with transplantation immunity.
OBJECTIVE: To study the roles of Foxp3 and IDO in mouse corneal allograft rejection.
METHODS: Routine penetrating keratoplasty was performed with C57BL/6 mice as donors and 30 BALB/c mice as recipients. The recipient mice were randomly divided into normal group (n=6), allograft group (intraperitoneal injection of normal saline solution for 6 times at 0, 2, 4, 6, 8 and 10 days after allograft, n=12) and dexamethasone group (intraperitoneal injection of 10 mg/kg dexamethasone, n=12). The graft rejection was evaluated under slit lamp microscope. Expression level of interferon-γ in grafts was detected by immunohistochemical staining. The expression levels of Foxp3 and IDO mRNA in grafts were detected by real-time PCR.
RESULTS AND CONCLUSION: The mean graft survival time in the allograft group was (14.833±1.472) days, and that in the dexamethasone group was (20.667±1.033) days (P < 0.05). The expression levels of interferon-γ as well as Foxp3 and IDO mRNA in grafts were significantly increased in the allograft group compared with the dexamethasone group (P < 0.05) at the 14th day postoperatively. These results suggest that Foxp3, IDO and interferon-γ play important roles in corneal allograft rejection; therefore, decreasing the expression levels of Foxp3, IDO and interferon-γ may contribute to inducing tolerance.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Interferon-gamma, Corneal Transplantation, Graft Rejection, Tissue Engineering

CLC Number:

R318

Gong Yu-bo1, Liu Yong2, Zhao Hong-wei1, Xu Qian-qian1, Guo Hui-ling1 . Expression levels of Foxp3 and indoleamine 2,3-dioxygenase in mouse corneal allograft rejection[J]. Chinese Journal of Tissue Engineering Research, 2018, 22(28): 4513-4517.

Figures/Tables 1

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