Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (46): 8620-8624.doi: 10.3969/j.issn.2095-4344.2012.46.014

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Primary culture, cryopreservation and resuscitation of human gingival fibroblasts

Hui Min1, Ni Ying2, Zheng Jing3, Liu Jian4, Wang Shuai5, Zhang Xiao-ming6   

  1. 1Department of Stomatology, 5Department of Radiology, 6Department of Prosthodontics and Orthodontics, Affiliated Hospital of Binzhou Medical College, Binzhou 256600, Shandong Province, China; 2School of Stomatology, 3Clinical Test Registration Center, Binzhou Medical College, Binzhou 256603, Shandong Province, China; 4Department of Orthodontics, the Hospital of Traditional Chinese Medicine in Rizhao, Rizhao 276800, Shandong Province, China
  • Received:2012-01-05 Revised:2012-04-06 Online:2012-11-11 Published:2013-03-16
  • Contact: Zhang Xiao-ming, Department of Prosthodontics and Orthodontics, Affiliated Hospital of Binzhou Medical College, Binzhou 256600, Shandong Province, China
  • About author:Hui Min, Experimentalist, Department of Stomatology, Affiliated Hospital of Binzhou Medical College, Binzhou 256600, Shandong Province, China

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Abstract:

BACKGROUND: Human gingival fibroblasts (HGFs) are the main cells in the connective tissue layers, which play an important role in many physiological and pathological processes.
OBJECTIVE: To perform primary culture, identification, cryopreservation and resuscitation for HGFs.
METHODS: First, the HGFs were cultured by the methods of tissue culture and identified by morphological and immunocytochemical analysis. Next, HGFs were frozen and resuscitated. And then their morphological changes were observed by the inverted microscope.
RESULTS AND CONCLUSION: The success rate of HGFs from the primary cells was 86.7%, and the HGFs showed fusiform or spindle-shaped. The immunochemistry study indicated that vimentin showed positive staining; while cytokeratin showed negative staining, which originated from mesoblastma. The cryopreservation and resuscitation of HGFs were successful. The biological character of HGFs after second passage was similar to that of the original generation. These results suggest that the primary culture, cryopreservation and resuscitation of HGFs can be feasible by using the method of tissue culture.

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