Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (11): 2009-2012.doi: 10.3969/j.issn.1673-8225.2011.11.027

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Expressions of osteoprotegrin and Wnt-1 in periodontal tissues under pressure force

Qin Ming-qun1, Xu Lian-jing1, Mao Jun-wu1, Huang Sheng-fu2   

  1. 1Affiliated Hospital of Guilin Medical College, Guilin  541001, Guangxi Zhuang Autonomous Region, China
    2Department of Orthodontics, Stomatological Hospital of Wuhan University, Wuhan  430079, Hubei Province, China
  • Received:2010-10-26 Revised:2011-01-14 Online:2011-03-12 Published:2011-03-12
  • Contact: Xu Lian-jing, Studying for master’s degree, Affiliated Hospital of Guilin Medical College, Guilin 541001, Guangxi Zhuang Autonomous Region, China xulianjing825@163.com
  • About author:Qin Ming-qun★, Master, Professor, Master’s supervisor, Affiliated Hospital of Guilin Medical College, Guilin 541001, Guangxi Zhuang Autonomous Region, China qmq@vip.sina.com
  • Supported by:

     the Key Science and Technology Item of Health Department of Guangxi, No. zhong200980*

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Abstract:

BACKGROUND: Current research found Wnt signaling pathways and related factors have the adjustment function for bone tissue. Wnt-1 is closely related with bone formation, but there was no report regarding whether Wnt-1 is correlated with orthodontic bone renovation.
OBJECTIVE: To investigate the changes of osteoprotegrin (OPG) and Wnt-1 expression by periodontal tissue during orthodontic tooth movement in rats.
METHODS: A total of 80 male Wistar rats were prepared for orthodontic tooth movement models and drawn materials at 1, 3, 7, 14 days, respectively. Every rat’s contralateral tooth served as control group. The morphology of periodontal tissues at pressure side were observed by hematoxylin-eosin staining; the Wnt-1 and OPG expressions at pressure side of periodontal tissue were determined by RT-PCR and immunohistochemical method.
RESULTS AND CONCLUSION: RT-PCR and immunohistochemical results demonstrated that, OPG was expressed at the pressure side at 1, 3, 7, and 14 days, but the expression was enhanced at 3 and 7 days (P < 0.05). Wnt-1 could be seen in the immunohistochemical results, which expressed stronger at 3 days compared with the control group (P < 0.05). It is confirmed that in orthodontic force, OPG participates in the process of orthodontic periodontal tissue rebuilding, OPG expression increased in press area in a time-dependent manner. Correlation between Wnt-1 and orthodontic bone rebuilt need to be further researched.

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