Abstract:

BACKGROUND: A large number of experiments have shown that the donor lung tissue and pulmonary vascular function can be stored under appropriate conditions. When the donor heart beating stops, the pulmonary gas exchange system can be tolerant to the warm ischemia for 60 minutes.
OBJECTIVE: To establish a simple and effective rat lung transplantation model with non-heart beating donor.
METHODS: Sixty Sprague-Dawley rats were randomly divided into heart beating donor group, non-heart beating donor-ischemia 30 minutes group and non-heart beating donor-ischemia 60 minutes group, there were 20 rats in each group. The lungs of the donor rats in the heart beating donor group were obtained and stored in 4 ℃ low potassium dextran solution for 4 hours; rats in the non-heart beating donor-ischemia 30 minutes group were bloodletting sacrificed, and the lungs of the rats were maintained ventilation for 30 minutes, and then performed with cold preservation after hot ischemia for 30 minutes; the treatment method in the non-heart beating donor-ischemia 60 minutes group was similar to that in the heart beating donor-ischemia 30 minutes group, the difference was that the time of hot ischemia. The peak airway pressure and pulmonary venous blood oxygen partial pressure was measured at 1, 15, 30, 45 and 60 minutes after the connection of the circulation loop in each group. After perfusion, the wet weight and dry weight ratio and the malondialdehyde level were detected.
RESULTS AND CONCLUSION: There was no significant difference of all the indexes between heart beating donor group and non-heart beating donor-ischemia 30 minutes group (P > 0.05). The peak airway pressure in each group was increased gradually along with the extension of time, but the peak airway pressure in non-heart beating donor-ischemia 60 minutes group was higher than that in the heart beating donor group and non-heart beating donor-ischemia 30 minutes group (P < 0.05). The pulmonary blood oxygen partial pressure in each group was gradually decreased with the extension of time, and the pulmonary blood oxygen partial pressure non-heart beating donor-ischemia 60 minutes group was lower than that in heart beating donor group and non-heart beating donor-ischemia 30 minutes group (P < 0.05). The wet/dry weight ratio and the malondialdehyde level in non-heart beating donor-ischemia 60 minutes group were higher than those in heart beating donor group and non-heart beating donor-ischemia 30 minutes group (P < 0.05). Non-heart beating donor-ischemia 30 minutes is a safe and effective method for lung transplantation.