Three-dimensional structure and morphology of the mandible in type 1 diabetes mellitus mice

doi:10.3969/j.issn.2095-4344.2013.28.001

Abstract

Abstract:

BACKGROUND:Diabetes mellitus is one of the most common systemic diseases, which often leads to the changes of the jaw and other bone structure, as well as the abnormal changes of mineral metabolism.

OBJECTIVE: To observe the three-dimensional structure and histopathological changes of the mandible in type 1 diabetes mellitus mice.

METHODS: The mice were randomly divided into control group and diabetes mellitus group. The diabetes mellitus group received intraperitoneal injection of 50 mg/kg streptozotocin for 5 days to establish a type 1 diabetes mellitus model, and the control group received intraperitoneal injection of citrate buffer.

RESULTS AND CONCLUSION: At 3 weeks after modeling, the micro-CT technique was used to observe the three-dimensional structure of the mandibles in the two groups. The quantitative analysis on the microstructure of cancellous bone and cortical bone showed that the bone mineral density, bone volume fraction, trabecular number and trabecular thickness of cancellous bone in the interest region in the mandible of type 1 diabetes mellitus mice were significantly decreased when compared with that in the control group (P < 0.01, P < 0.05), while the structure model index was increased significantly (P < 0.05); the mineral density and area of cortical bone were decreased in the diabetes mellitus group (P < 0.05). Hematoxylin-eosin staining showed that the number and volume of mandibular trabeculae of type 1 diabetes mellitus mice were decreased. The results suggest that the three-dimensional structure of the cancellous bone and cortical bone in the streptozotocin-induced type 1 diabetes mellitus mice are changed significantly, and the microstructure change of the cancellous bone is more obvious.

Key words: tissue construction, bone tissue construction, type 1 diabetes mellitus, mandibular bone structure, cancellous bone, cortical bone, bone microstructure, bone morphometry, micro-CT, histological section, provincial grants-supported paper

CLC Number:

R318

Zhang Jun, Jiang Li-ting, Wang Jin-shen, Zhou Qi, Gao Yi-ming1. Three-dimensional structure and morphology of the mandible in type 1 diabetes mellitus mice[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(28): 5101-5107.

Figures/Tables 6

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