Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (46): 8563-.doi: 10.3969/j.issn.1673-8225.2010.46.004

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Comparison of tenocytes isolated by tissue block culture and enzyme digestion

Shen Xiao-tao 1, 2, 3, 4, Chen Si-yun 1, 2, 3, Zhao Bao-yin 1, 2, 3, Zheng Xin 1, 2, 3 , Cai Dong-qing 1, 2, 3, 4   

  1. 1Key Laboratory for Regenerative Medicine, Ministry of Education, 2Jointed Laboratory for Regenerative Medicine, the Chinese University of Hong Kong&Jinan University, 3the International Base of Collaboration for Science & Technology, the Ministry of Science and Technology & Guangdong Province Science and Technology Bureau, 4Department of Biomedical Engineering, Jinan University, Guangzhou  510632, Guangdong Province, China
  • Online:2010-11-12 Published:2010-11-12
  • Contact: Cai Dong-qing, Professor, Doctoral supervisor, 1Key Laboratory for Regenerative Medicine, Ministry of Education, 2Jointed Laboratory for Regenerative Medicine, the Chinese University of Hong Kong&Jinan University, 3the International Base of Collaboration for Science & Technology, the Ministry of Science and Technology & Guangdong Province Science and Technology Bureau, 4Department of Biomedical Engineering, Jinan University, Guangzhou 510632, Guangdong Province, China tdongbme@jnu.edu.cn
  • About author:Shen Xiao-tao, Assistant Technician, 1Key Laboratory for Regenerative Medicine, Ministry of Education, 2Jointed Laboratory for Regenerative Medicine, the Chinese University of Hong Kong&Jinan University, 3the International Base of Collaboration for Science & Technology, the Ministry of Science and Technology & Guangdong Province Science and Technology Bureau, 4Department of Biomedical Engineering, Jinan University, Guangzhou 510632, Guangdong Province, China tsxt@jnu.edu.cn
  • Supported by:

    the National High-Tech Research and Development Program of China (863 Program), No. 2007AA02Z105*; the National Natural Science Foundation of China, No. 30770886*, 30570369*, 30340038*, 30973158*; the Natural Science Foundation of Guangdong Province, No. 04105826*; the Tackle Key Program in Science and Technology of Guangdong Province, No. 2004B30601007*

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Abstract:

BACKGROUND: It is importance to activate tenocytes groups to repair the injured ligament and tendon. So, separating the different groups of tenocytes will elucidate the maintenance and repair of normal ligament and tendon structures.
OBJECTIVE: To compare the cell morphology, proliferation, replicative senescence and the cell surface markers expression of tenocytes isolated by tissue block culture and enzyme digestion.
METHODS: Tenocytes obtained from patellar tendon were isolated by tissue block culture and enzyme digestion. The cell morphology, proliferation, replicative senescence and the cell surface markers of isolated tenocytes were investigated.  
RESULTS AND CONCLUSION: There were significant differences between tenocytes isolated by tissue block culture and enzyme digestion in cell morphology, proliferation, replicative senescence and the cell surface markers. It suggested that tenocytes of patellar tendon might include different groups and the individual group of tenocytes may play the unique function in normal tendon function and tendon healing.

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