Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (46): 8616-.doi: 10.3969/j.issn.1673-8225.2010.46.016

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Effect of puerarin combined with estradiol on bone tissues and bone metabolism in ovariectomized rats

Li Hai, Wang Jin-hua, Huang Hai-ling, Li Biao   

  1. Department of Histology and Embryology, Youjiang Medical College for Nationalities, Baise 533000, Guangxi Zhuang Autonomous Region, China
  • Online:2010-11-12 Published:2010-11-12
  • About author:Li Hai, Lecturer, Department of Histology and Embryology, Youjiang Medical College for Nationalities, Baise 533000, Guangxi Zhuang Autonomous Region, China lihaiying702@163.com
  • Supported by:

    the Natural Science Foundation of Guangxi Zhuang Autonomous Region, No. [2010]13*; the Research Foundation of Youjiang Medical College for Nationalities, No. yk2009002*

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Abstract:

BACKGROUND: Chinese medication combined with estradiol can be used to treat osteoporosis, which may reduce estradiol dose, decrease the adverse reaction and complication.
OBJECTIVE: To observe the effects of small dose of puerarin combined with estradiol on bone tissues and bone metabolism in ovariectomized rats.
METHODS: 5-month-old healthy female rats were divided into five experimental groups: sham-operated, model, puerarin, estradiol and low-dose puerarin + estradiol groups. All rats were prepared for ovariectomized models except those in the sham-operated group. Totally 100 μg/kg estradiol were subcutaneous injected into rats, twice per week, or 25 mg/kg puerarin were injected, once per day. The bone tissues were observed and the serum calcium, phosphorus and alkaline phosphatase (ALP) levels were measured at 4, 8, 12 and 20 weeks after operation. 
RESULTS AND CONCLUSION: Compared with the sham-operated group, the serum calcium and phosphorus of the model group was decreased significant at 4 and 8 weeks after operation (P < 0.01), but the ALP levels were notably increased (P < 0.01), the bone tissues presented with osteoporosis. Compared with the sham-operated group, there were no significances among the three medication groups in the serum calcium, phosphorus and ALP levels (P > 0.05). In addition, there was no significance intergroup comparison among three treatment groups (P > 0.05). The small dose of puerarin + estradiol shows similar effects to large single doses of puerarin or estradiol on the treatment of rat ovariectomized osteoporosis.

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