Biocompatibility and in vitro osteogenic differentiation of alginates/chitosan/58S bioglass composite membrane for guided bone regeneration

doi:10.12307/2023.508

Abstract

Abstract: BACKGROUND: Natural biopolymers have excellent biodegradability. Their structures are similar to natural extracellular matrix, which is conducive to tissue repair. Natural biopolymers have a great potential in developing new absorbable membranes.
OBJECTIVE: To develop a new absorbable membrane for guided bone regeneration by incorporating 58S bioglass powders into alginate/chitosan composites and study the physical, chemical and biological properties.
METHODS: Alginate/chitosan/bioglass composite membranes were prepared according to the concentration gradient of 0, 5, 7.5 and 10 g/L 58S bioglass. The groups were recorded as 0BG, 0.5BG, 0.75BG and 1BG composite membranes according to the concentration gradient. The morphology of each group of composite membranes was characterized by metallurgical microscope and scanning electron microscope. The physiochemical properties of the resultant polymers were assessed via Fourier transform infrared spectroscopy and swelling ratio in each group. The biological properties of the composite membranes were compared by in vitro degradation experiments, protein adsorption experiments, cell adhesion experiments, cell proliferation experiments and alkaline phosphatase activity tests in each group.
RESULTS AND CONCLUSION: (1) Metallurgical microscope and scanning electron microscope showed that samples of each group had the typical porous structure of hydrogel, and the bioglass particles were embedded in the pores of the composite membrane. The porosity of all composite membranes was greater than 90%. The sequence was: 0BG > 0.5BG > 0.75BG > 1BG. (2) Fourier transform infrared spectroscopy exhibited the characteristic peaks of the polymer formed by alginate and chitosan and the evidence of the existence of bioglass in the polymer. The composite membranes with 58S bioglass powders showed certain advantages in swelling ratio, in vitro degradation and biological activity, and this advantage was more obvious with the increase of bioglass content. In particular, 0.75BG and 1BG composite membranes could exhibit a high level of osteogenic differentiation over a long period of time. (3) These results suggested that in the experimental research range, when the mass concentration of 58S bioglass was 7.5 and 10 g/L, the alginate/chitosan/bioglass composite membranes can achieve relatively excellent biological activity, and it has the potential to be applied in the study of guided bone regeneration.

Key words: guided bone regeneration, absorbable membrane, natural biopolymers, alginates, chitosan, bioglass

CLC Number:

He Fan, Shan Xianfeng, Xiong Xiuli, Wang Xuejin. Biocompatibility and in vitro osteogenic differentiation of alginates/chitosan/58S bioglass composite membrane for guided bone regeneration[J]. Chinese Journal of Tissue Engineering Research, 2023, 27(25): 3984-3991.

Figures/Tables 8

References

[1] Saghiri MA, Freag P, Fakhrzadeh A, et al. Current technology for identifying dental implants: a narrative review. Bull Natl Res Cent. 2021;45(1):1-11.
[2] Abdelaziz D, Hefnawy A, Al-Wakeel E, et al. New biodegradable nanoparticles-in-nanofibers based membranes for guided periodontal tissue and bone regeneration with enhanced antibacterial activity. J Adv Res. 2021;28:51-62.
[3] FLORJANSKI W, ORZESZEK S, OLCHOWY A, et al. Modifications of Polymeric Membranes Used in Guided Tissue and Bone Regeneration. Polymers (Basel). 2019;11(5):782.
[4] Kaessmair S, Distler T, Schaller E, et al. Identification of mechanical models and parameters for alginate‐based hydrogels as proxy materials for brain tissue. PAMM. 2021;20(1):e202000338.
[5] Lawson MA, Barralet JE, Wang L, et al. Adhesion and growth of bone marrow stromal cells on modified alginate hydrogels. Tissue Eng. 2004;10(9-10):1480-1491.
[6] Vo-An Q, Nguyen TC, Nguyen QT, et al. Novel Nanoparticle Biomaterial of Alginate/Chitosan Loading Simultaneously Lovastatin and Ginsenoside RB1: Characteristics, Morphology, and Drug Release Study. Int J Polym Sci. 2021. doi:10.1155/2021/5214510
[7] 吴广升,王亚男,惠光艳,等.加载miRNA-21壳聚糖基纳米粒对牙周膜干细胞成骨分化的影响[J].解放军医药杂志,2021,33(1):1-6.
[8] Zou Z, Wang L, Zhou Z, et al. Simultaneous incorporation of PTH(1-34) and nano-hydroxyapatite into Chitosan/Alginate Hydrogels for efficient bone regeneration. Bioact Mater. 2021;6(6):1839-1851.
[9] Lahiji A, Sohrabi A, Hungerford DS, et al. Chitosan supports the expression of extracellular matrix proteins in human osteoblasts and chondrocytes. J Biomed Mater Res. 2000;51(4):586-595.
[10] Karakeçili AG, Gümüşderelioğlu M. Physico-chemical and thermodynamic aspects of fibroblastic attachment on RGDS-modified chitosan membranes. Colloid Surface B. 2008;61(2):216-223.
[11] Florczyk SJ, Kim DJ, Wood DL, et al. Influence of processing parameters on pore structure of 3D porous chitosan-alginate polyelectrolyte complex scaffolds. J Biomed Mater Res A. 2011;98A(4): 614-620.
[12] Xu S, Chen X, Yang X, et al. Preparation and In Vitro Biological Evaluation of Octacalcium Phosphate/Bioactive Glass-Chitosan/Alginate Composite Membranes Potential for Bone Guided Regeneration. J Nanosci Nanotechno. 2016;16(6):5577-5585.
[13] Zhang Y, Zhang M. Synthesis and characterization of macroporous chitosan/calcium phosphate composite scaffolds for tissue engineering. J Biomed Mater Res. 2001;55(3):304-312.
[14] Luna SM, Silva SS, Gomes ME, et al. Cell adhesion and proliferation onto chitosan-based membranes treated by plasma surface modification. J Biomater Appl. 2011;26(1):101.
[15] Greenspan D. Bioglass at 50 - A look at Larry Hench’s legacy and bioactive materials. Biome Glass. 2019;5(1):178-184.
[16] Paramita P, Ramachandran M, Narashiman S, et al. Sol-gel based synthesis and biological properties of zinc integrated nano bioglass ceramics for bone tissue regeneration. J Mater Sci-Mater M. 2021;32(1):1-11.
[17] Pereira MM, Clark AE, Hench LL. Calcium phosphate formation on sol-gel-derived bioactive glasses in vitro. J Biomed Mater Res. 1994; 28(6):693-698.
[18] 曹钰彬,刘畅,潘韦霖,等.引导骨再生屏障膜改良的研究进展[J].华西口腔医学杂志,2019(3):325-329.
[19] 代香林,张文凤,姚喜军,等.钛酸钡/聚乳酸复合压电薄膜材料对 MC3T3-E1 细胞黏附、增殖和成骨分化能力的影响[J].中国组织工程研究,2023,27(3):367-373.
[20] 封小霞,侯玮玮,金晓婷,等.构建聚乳酸-羟基乙酸电纺丝-壳聚糖电喷微球牙周仿生膜[J].中国组织工程研究,2020,24(4):511-516.
[21] Luo K, Wang L, Wang Y, et al. Porous 3D hydroxyapatite/polyurethane composite scaffold for bone tissue engineering and its in vitro degradation behavior. Ferroelectrics. 2020;566(1):104-115.
[22] Zhang L, Guo J, Zhou J, et al. Blend membranes from carboxymethylated chitosan/alginate in aqueous solution. J Appl Polym Sci. 2000;77(3):610-616.
[23] Ho YC, Mi FL, Sung HW, et al. Heparin-functionalized chitosan-alginate scaffolds for controlled release of growth factor. Int J Pharmaceut. 2009;376(1-2):69-75.
[24] Sowjanya JA, Singh J, Mohita T, et al. Selvamurugan. Biocomposite scaffolds containing chitosan/alginate/nano-silica for bone tissue engineering. Colloid Surface B. 2013;109:294-300.
[25] Kavya KC, Jayakumar R, Nair S, et al. Fabrication and characterization of chitosan/gelatin/nSiO2 composite scaffold for bone tissue engineering. Int J Biol Macromol. 2013;59:255-263.
[26] Kaysinger KK, Ramp WK. Extracellular pH modulates the activity of cultured human osteoblasts. J Cell Biochem. 1998;68(1):83-89.
[27] Alsberg E, Kong HJ, Hirano Y, et al. Regulating Bone Formation via Controlled Scaffold Degradation. J Dent Res. 2003;82(11):903-908.
[28] Höhn S, Zheng K, Romeis S, et al. Effects of Medium pH and Preconditioning Treatment on Protein Adsorption on 45S5 Bioactive Glass Surfaces. Adv Mater Interfaces. 2020;7(15):2000420.
[29] Kilpadi KL, Chang PL, Bellis SL. Hydroxylapatite binds more serum proteins, purified integrins, and osteoblast precursor cells than titanium or steel. J Biomed Mater Res. 2001;57(2):258-267.