Constructing a vascular endothelial growth factor sustained-released multi-wall carbon nanotube composite scaffold

doi:10.3969/j.issn.2095-4344.2013.03.009

Abstract

Abstract:

BACKGROUND: The implantation of porcine small intestinal submucosa (PSIS) for abdominal wall defects has deficiency in early vascularization, which can result in the failure of abdominal wall reconstruction.
OBJECTIVE: To construct a multi-wall carbon nanotube (MWCNT)-PSIS composite scaffold which can sustained-release vascular endothelial growth factor (VEGF), and to evaluate the VEGF sustained-release performance, mechanical property and cytotoxicity of the composite scaffold in vitro.
METHODS: The VEGF-loaded MWCNT was integrated with two-layer PSIS by dip dyeing to construct the composite scaffold. According the different weight percents of MWCNT, five groups were divided: PSIS (0%), 1%, 3%, 5%, 10% MWCNT-PSIS.
RESULTS AND CONCLUSION: (1) The VEGF sustained-release property of the composite scaffolds: The accumulation concentration of each group increased with the extension of time, meanwhile the released concentration increased gradually with the increasing quality of the MWCNT. (2) The mechanical property of the composite scaffolds: The maximum load and elastic modulus of 1%, 3%, 5%, 10% MWCNT-PSIS groups were superior to original PSIS scaffold (control group) (P < 0.05). The maximum load increased gradually along with the increasing quality of the MWCNT. (3) The fibroblast cytotoxicity of the composite scaffolds: The composite scaffolds that contained ≤ 5% MWCNT were proved to have no significant influence on the fibroblast proliferation. The above results in vitro confirmed the composite scaffold we constructed possesses excellent VEGF sustained-released performance, improves mechanical property and promotes the endothelial cell proliferation.

Key words: biomaterials, nano-biological materials, vascular endothelial growth factor, multi-wall carbon nanotube, porcine small intestinal submucosa, composite scaffolds, abdominal wall defects, maximum loading, elastic modulus, cytotoxicity, provincial grants-supported paper, biomaterial photographs-containing paper

CLC Number:

R318

Feng Xue-yi, Liu Zheng-ni, Tang Rui, Hu Heng-yao. Constructing a vascular endothelial growth factor sustained-released multi-wall carbon nanotube composite scaffold[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(3): 433-439.

Figures/Tables 5

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