Chinese Journal of Tissue Engineering Research ›› 2013, Vol. 17 ›› Issue (3): 427-432.doi: 10.3969/j.issn.2095-4344.2013.03.008

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Effect of silk fibroin on the regulation of nano-hydroxyapatite crystal growth

Xu Ning1, Chen Yu-yun2, Zhong Jian2,  Xu Guo-hua1, Ye Xiao-jian1   

  1. 1 Department of Orthopedics, Affiliated Changzheng Hospital, Second Military Medical University of Chinese PLA, Shanghai 200003, China
    2 National Engineering Research Center for Nanotechnology, Shanghai 200241, China
  • Received:2012-05-18 Revised:2012-06-15 Online:2013-01-15 Published:2013-01-15
  • Contact: Corresponding author: Ye Xiao-jian, Professor, Department of Orthopedics, Affiliated Changzheng Hospital, Second Military Medical University of Chinese PLA, Shanghai 200003, China yexj1964@hotmail.com Corresponding author: Xu Guo-hua, Associate professor, Department of Orthopedics, Affiliated Changzheng Hospital, Second Military Medical University of Chinese PLA, Shanghai 200003, China xuguohuamail@163.com
  • About author:Xu Ning★, Studying for master’s degree, Department of Orthopedics, Affiliated Changzheng Hospital, Second Military Medical University of Chinese PLA, Shanghai 200003, China xu582@hotmail.com Chen Yu-yun★, Master, National Engineering Research Center for Nanotechnology, Shanghai 200241, China

Abstract:

BACKGROUND: Silk fibroin (SF) content has been reported to have great influences on the properties and structure of SF/nano-hydroxyapatite (SF/n-HA) complex in previous studies.
OBJECTIVE: To observe the effect of SF content on the crystal growth of n-HA.
METHODS: SF/n-HA complex was prepared using the method of physical chemistry. The effect of SF content (0, 10%, 20%, 30%, 40%) on the crystal growth of n-HA was studied using thermogravimetric analysis, Fourier transform infrared spectroscopy, transmission electron microscope, and X-ray powder diffractometer.
RESULTS AND CONCLUSION: The results show that SF affected the crystal nucleation and growth of n-HA obviously. SF induced n-HA crystals to preferentially grow along the c axis, the n-HA aspect ratio increased, and n-HA morphology changed from rod-like to needle-like. When SF content was 10% and 20%, n-HA crystals radically dispersed in SF matrix orderly; when SF content was 30% and 40 %, the n-HA crystals were disordered in SF matrix. These findings indicate that 30% SF is the critical binding percent in the SF/n-HA preparation.

Key words: biomaterials, nano-biological materials, nano-hydroxyapatite, silk fibroin, crystal nucleation, crystal morphology, the National 973 Program of China, biomaterial photographs-containing paper

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