Epigallocatechin gallate alleviates skeletal muscle ischemia-reperfusion injury in rats

doi:10.12307/2021.112

Abstract

Abstract:

BACKGROUND: Skeletal muscle ischemia-reperfusion (I/R) injury is a serious problem commonly encountered in clinical practice which seriously impacts the function and survival rate of replanted limbs. Epigallocatechin gallate (EGCG) has been proved to be antioxidant, anti-inflammatory and anti-apoptotic.

OBJECTIVE: To investigate the role of EGCG in I/R injury of rat skeletal muscle.
METHODS: Thirty male Sprague-Dawley rats were randomly divided into five groups: sham operation group, I/R group, and three EGCG groups in which 10, 100 and 200 mg/kg EGCG was intraperitoneally injected 30 minutes before reperfusion respectively according to the weight of the rats. A lower limb I/R injury model was made in each group except for the sham operation group. The levels of tumor necrosis factor-α and interleukin-1β in serum were measured and the levels of superoxide dismutase and myeloperoxidasein gastrocnemius muscle tissue were detected. Morphological changes of gastrocnemius muscle tissue were observed by hematoxylin-eosin staining. Cell apoptosis in gastrocnemius muscle tissue was observed by the TUNEL assay.
RESULTS AND CONCLUSION: In the 100 and 200 mg/kg EGCG groups compared to the I/R group, there were the decreased levels of tumor necrosis factor-α and interleukin-1β in serum and myeloperoxidase in gastrocnemius muscle tissue and the increased level of superoxide dismutase in gastrocnemius muscle tissue, which were positively correlated with the EGCG dose. Hematoxylin-eosin staining results suggested an evident improvement in pathological manifestations ofgastrocnemius muscle, such as the continuity and integrity of muscle fibers and the degree of interstitial edema, in 100 and 200 mg/kg EGCG groups. TUNEL assay showed that 100 and 200 mg/kg EGCG inhibited cell apoptosis in gastrocnemius muscle tissue. To conclude, EGCG protects rat skeletal muscle from I/R injury by reducing inflammatory reaction, antioxidant stress and inhibiting apoptosis.

Key words: ischemia-reperfusion injury, skeletal muscle, epigallocatechin gallate, oxidative stress, inflammation, apoptosis, rat

CLC Number:

Xue Qing, Tong Liangcheng, Yang Zhiwei, Wang Jianling, Zhao Lei, Zhou Sheng, Peng Sai, Li Ying. Epigallocatechin gallate alleviates skeletal muscle ischemia-reperfusion injury in rats[J]. Chinese Journal of Tissue Engineering Research, 2021, 25(26): 4145-4149.

Figures/Tables 4

References

[1] 李颖,孙中洋,闫浩,等.白果内酯对大鼠骨骼肌细胞缺血再灌注损伤的作用[J].中华创伤杂志,2020,36(7):659-663.
[2] WANG XT, TIAN Y, XU WX, et al. Protective Effects of Modeled Superoxide Dismutase Coordination Compound (MSODa) Against Ischemia/Reperfusion Injury in Rat Skeletal Muscle. Cell PhysiolBiochem. 2015;37(2):465-476.
[3] LI Y, JIANG J, TONG L, et al. Bilobalide protects against ischemia/reperfusion-induced oxidative stress and inflammatory responses via the MAPK/NF-κB pathways in rats. BMC MusculoskeletDisord. 2020;21(1):449.
[4] 阮思杰,阳富春,阳茂春,等.骨骼肌缺血后调适动态pH测量及模拟酸灌注减轻缺血再灌注损伤[J].中国组织工程研究,2017,21(16):2558-2564.
[5] DU JT, LI W, YANG JY, et al. Hydrogen sulfide is endogenously generated in rat skeletal muscle and exerts a protective effect against oxidative stress. Chin Med J (Engl). 2013;126(5):930-836.
[6] ZHU L, HUANG Y, PEI G. Study of L-arginine-nitric oxide pathway in ischemia-reperfusion injured limbs in rats. Chin J Traumatol. 2002;5(1):16-20.
[7] 姜洪军,任晓平.细胞凋亡与骨骼肌缺血/再灌注损伤[J].医学综述, 2017,23(10):1898-1902.
[8] HONG Y, ZHANG B, YU L, et al. Cell membrane integrity and revascularization: The possible functional mechanism of ischemic preconditioning for skeletal muscle protection against ischemic-reperfusion injury. ActaHistochem. 2017;119(3):309-314.
[9] 朱斌,宋航,陈晓光,等.用商品茶多酚制备高纯度表没食子儿茶素没食子酸酯的工艺研究[J].华中师范大学学报(自然科学版),2008,42(4): 574-577.
[10] 王丽,林昕,汪禄祥,等.茶叶中多种功能成分含量测定与分析研究[J].食品安全质量检测学报,2019,10(22):7779-7786.
[11] 赵峰,阙付有,梁京,等.儿茶素单体EGCG酶法修饰研究及其产物抗氧化活性评价[J].天然产物研究与开发,2015,27(3):490-495,533.
[12] 彭沙沙,夏丰,王进,等.三氯化钆对Sprague Dawley大鼠肝脏缺血再灌注损伤的影响及作用机制[J].中华肝胆外科杂志,2020,26(3):213-217.
[13] 卢中山,王伟,钟自彪,等.肝脏缺血再灌注损伤干预措施的研究进展[J].中华肝胆外科杂志,2019,25(11):871-874.
[14] 张铂,王兵,曹书华,等.表没食子儿茶素没食子酸酯对大鼠创伤性脑水肿保护作用的研究[J].中国中西医结合急救杂志,2015,22(4):402-407.
[15] 张玉琴,朱旭强,吴力新,等.表没食子儿茶素没食子酸酯对脑损伤小鼠认知和氧化应激的影响[J].中华实验外科杂志,2018,35(3):475-477.
[16] LI YW, ZHANG Y, ZHANG L, et al. Protective effect of tea polyphenols on renal ischemia/reperfusion injury via suppressing the activation of TLR4/NF-κBp65 signal pathway. Gene. 2014;542(1):46-51.
[17] KOCMAN EA, OZATIK O, SAHIN A, et al. Effects of ischemic preconditioning protocols on skeletal muscle ischemia-reperfusion injury. J Surg Res. 2015; 193(2):942-952.
[18] SIMON MA, TIBBITS EM, HOAREAU GL, et al. Lower extremity cooling reduces ischemia-reperfusion injury following Zone 3 REBOA in a porcine hemorrhage model. J Trauma Acute Care Surg. 2018;85(3):512-518.
[19] 李林,胡海姣,刁玉刚.损伤控制性复苏对低温失血性休克大鼠全脑缺血-再灌注损伤保护作用[J].创伤与急危重病医学,2019,7(2):82-84,87.
[20] 张艳平,张倩,阎玮,等.浅低温对大鼠脑缺血再灌注时皮质Mfn2表达的影响[J].中华麻醉学杂志,2019,39(11):1383-1386.
[21] 肖靖远,林飞,潘灵辉,等.地塞米松调控PI3K/AKT通路减轻肺缺血/再灌注损伤的作用及机制[J].中华危重病急救医学,2020,32(2):188-193.
[22] 施源,李宏军,王君君,等.血必净通过激活PI3K/Akt/mTOR信号通路减轻大鼠睾丸缺血再灌注损伤[J].中国病理生理杂志,2020,36(2): 329-335.
[23] 胡敏,於四军,刘惠亮.褪黑素对大鼠挤压综合征模型骨骼肌缺血再灌注损伤的影响[J].中国循环杂志,2017,32(z1):42.
[24] 王婷,冷玉芳,陈平,等.青藤碱对大鼠肢体缺血再灌注损伤及骨骼肌细胞Bcl-2和Bax表达的影响[J].中华麻醉学杂志,2016,36(9):1094-1097.
[25] 申震,朱付平,刘天举,等.桃红四物液对大鼠肢体缺血-再灌注损伤炎症因子表达的影响研究[J].中华中医药学刊,2018,36(2):374-377.
[26] RUZICKA J, URDZIKOVA LM, SVOBODOVA B, et al. Does combined therapy of curcumin and epigallocatechin gallate have a synergistic neuroprotective effect against spinal cord injury? Neural Regen Res. 2018;13(1):119-127.
[27] 王金妮,欧冰凝,梁钢.表没食子儿茶素没食子酸酯全乙酰化衍生物的合成及急性毒性试验[J].广西医学,2012,34(2):129-133.
[28] 李霄,张玄,王权成,等.儿茶素没食子酸酯预处理对大鼠肝脏缺血再灌注损伤的保护作用[J].中华肝胆外科杂志,2018,24(3):199-203.
[29] 丁华胜,王永剑,黄忠毅.丹参酮Ⅱ A对大鼠心肌缺血再灌注损伤中HMGB1、IL-1β表达及SOD水平的影响[J].中国中医急症,2020,29(5): 776-778,801.
[30] 周光居,张茂,干建新,等.MicroPET及MPO对大鼠ALI时肺内炎症反应评估效果的研究[J].中华急诊医学杂志,2014,23(4):399-401.
[31] 梁倩倩,田华,庄宝祥,等.脉络宁对兔肢体缺血/再灌注损伤过程中SOD及MPO的影响[J].中国药理学通报,2012,28(6):885-886.