Abstract: BACKGROUND: Studies have found that drugs that block the secretion of inflammatory transmitters in the synovium have good application prospects in the treatment of acute gouty arthritis. In recent years, great achievements have been made in the treatment of acute gouty arthritis with traditional Chinese medicine.
OBJECTIVE: To study the effect of alcohol extract of Gardneria angustifolia on serum inflammatory factors and synovial cell function in patients with acute gouty arthritis.
METHODS: Sixty healthy Sprague-Dawley rats were randomly divided into six groups: blank control group, model control group, colchicine treatment group, Gardneria angustifolia alcohol extract high, medium and low dose treatment groups, withy 10 rats in each group. In addition to the blank control group, the other groups were injected with sodium urate solution into the articular cavity to prepare acute gouty arthritis models, and then treated with colchicine, high, medium and low doses of Gardneria angustifolia alcohol extract. The swelling degree of the joint was observed at 1-7 days after modeling. At 7 days after modeling, the levels of interleukin-1β, tumor necrosis factor-α, interleukin-17 in the serum and synovium were detected by ELISA; the pathological changes of the synovium were observed by hematoxylin-eosin staining, and the expression of nuclear factor- κB, ERK1/2 and JNK protein in the synovium was detected by western blot.
RESULTS AND CONCLUSION: In the model control group, the joint swelling, the level of interleukin-1β, tumor necrosis factor-α, and interleukin-17 in the serum and synovium were increased, the synovium had obvious inflammatory cell infiltration and synovium cell proliferation, the expression of nuclear factor-κB, ERK1/2 and p-JNK protein was significantly increased. After the treatment with colchicine and Gardneria angustifolia alcohol extracts, the above indexes were significantly improved, and there was no significant difference in the high dose Gardneria angustifolia alcohol extract group and colchicine group. Moreover, Gardneria angustifolia alcohol extracts showed a dose-dependent effect. The alcohol extract of Gardneria angustifolia can significantly improve the symptoms of acute gouty arthritis in rats, down-regulate the expression of inflammatory factors, and improve the function of synovial cells, which may be related to the down-regulation of nuclear factor-κB, ERK1/2 and p-JNK protein expression.

Key words: bone, gouty arthritis, Gardneria angustifolia alcohol extract, inflammation, factor, synovium, rat, experiment