Mechanism by which allicin inhibits proliferation and migration but promotes apoptosis of human fibroblasts

doi:10.3969/j.issn.2095-4344.2354

Abstract

Abstract:

BACKGROUND: Allicin has an anti-fibrosis effect and can inhibit the proliferation and collagen synthesis of hyperproliferation of fibroblasts, but the exact mechanism of allicin inhibiting the proliferation and inducing the apoptosis of fibroblasts remains unclear.

OBJECTIVE: To explore the mechanism of allicin inhibiting proliferation, migration and promoting apoptosis of human fibroblasts, providing new ideas for prevention and treatment of epidural adhesions.

METHODS: Logarithmic fibroblasts from human epidural scar were treated by allicin at a gradient of concentration for 24 hours. Cell morphology was observed using a fluorescence microscope. Cell counting kit-8 assay, scratch assay and flow cytometry were used to measure cell proliferation, migration and apoptosis, respectively, while western blot and real-time quantitative PCR were used to measure the expression levels of proliferating cell nuclear antigen, Bcl-2 and Bax related to cell proliferation and apoptosis.

RESULTS AND CONCLUSION: After treated by allicin for 2 hours, the fibroblasts shrunk, deformed and became smaller in size. Their nuclei were retracted and fragmented. Cell viability and migration rate decreased significantly in a dose-dependent manner (P < 0.01). The percentage of G₁ phase cells significantly increased (P < 0.01), while that of S phase and G₂ phase cells significantly decreased in a dose-dependent manner (P < 0.01). Similarly, the percentage of apoptotic cells increased significantly in a dose-dependent manner (P < 0.01). Compared with control group, the expression levels of proliferating cell nuclear antigen and Bcl-2 significantly decreased (P < 0.01), while the Bax level significantly increased (P < 0.01). Findings from this study indicate that allicin can inhibit the human fibroblast proliferation, migration and induce its apoptosis, possibly by regulating the expression of proliferating cell nuclear antigen, Bcl-2 and Bax.

Key words: cell, fibroblast, allicin, epidural, scar, fibrosis, collagen, gene

CLC Number:

Zhu Shoulei, Yang Jiandong, Cai Jun, Zhang Yujie, Tian Yuan. Mechanism by which allicin inhibits proliferation and migration but promotes apoptosis of human fibroblasts[J]. Chinese Journal of Tissue Engineering Research, 2020, 24(35): 5662-5667.

Figures/Tables 10

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