Baicalein inhibits the biological behavior of hepatocellular
carcinoma stem cells by downregulation of Decoy receptor 3 expression

doi:10.3969/j.issn.2095-4344.2017

Chinese Journal of Tissue Engineering Research ›› 2020, Vol. 24 ›› Issue (7): 1023-1029.doi: 10.3969/j.issn.2095-4344.2017

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Baicalein inhibits the biological behavior of hepatocellular carcinoma stem cells by downregulation of Decoy receptor 3 expression

Cen Yanhui^{1, 2, 3}, Xia Meng¹, Jia Wei¹, Luo Weisheng¹, Lin Jiang¹, Chen Songlin², Chen Wei³, Liu Peng¹, Li Mingxing¹, Li Jingyun¹, Li Manli¹, Ai Dingding¹, Jiang Yunxia¹

¹Guangxi University of Chinese Medicine, Nanning 530001, Guangxi Zhuang Autonomous Region, China; ²First Affiliated Hospital of Guangxi University of Chinese Medicine, Nanning 530023, Guangxi Zhuang Autonomous Region, China; ³Guangxi Key Laboratory of Basic Research in Traditional Chinese Medicine, Guangxi University of Chinese Medicine, Nanning 530001, Guangxi Zhuang Autonomous Region, China

Received:2018-12-20 Revised:2018-12-27 Accepted:2019-03-12 Online:2020-03-08 Published:2020-01-19
Contact: Jia Wei, Master, Associate professor, Guangxi University of Chinese Medicine, Nanning 530001, Guangxi Zhuang Autonomous Region, China Luo Weisheng, Professor, Guangxi University of Chinese Medicine, Nanning 530001, Guangxi Zhuang Autonomous Region, China
About author:Cen Yanhui, MD, Professor, Guangxi University of Chinese Medicine, Nanning 530001, Guangxi Zhuang Autonomous Region, China Xia Meng, MD, Associate professor, Guangxi University of Chinese Medicine, Nanning 530001, Guangxi Zhuang Autonomous Region, China Cen Yanhui and Xia Meng contributed equally to this work.
Supported by:
the National Natural Science Foundation of China, No. 8166150270; China Postdoctoral Foundation, No. 2017M6232; Guangxi Specialized Expert Team, No. GZKZ-G1103; the Special Project of Traditional Chinese Medicine Science and Technology of Health Department of Guangxi Zhuang Autonomous Region, No. GZBZ16-07 and GZLC16-23; High-Level Talent Team Cultivation Project of Qi Huang Engineering in Guangxi University of Chinese Medicine, No. 2018001; Open Research Project of Guangxi Key Laboratory of Basic Research in Traditional Chinese Medicine, No. 17-259-49-09; Guangxi First-Class Discipline Construction Project, No. 2018XK023 and 2018XK024 and 2019CK137; Guangxi Zhuang-Yao Pharmaceutical Key Laboratory Construction Project, No. GXZYKF-201708; Open Research Project of Guangxi Key Laboratory of Basic Research in Traditional Chinese Medicine; Young and Middle-aged Teachers Fundamental Ability Enhancement Project in Guangxi Universities, No. 2019KY0327; Scientific Research Project of Guangxi University of Chinese Medicine in 2019, No. 2019MS001

Abstract

Abstract:

BACKGROUND: To date, there is no report on the anti-hepatoma mechanism of Chinese herbal medicine targeting hepatocellular carcinoma stem cells; therefore, relevant research is necessary.

OBJECTIVE: To observe the effect of baicalein on the expression of Decoy receptor 3 in hepatocellular carcinoma stem cells as well as the biological behavior of hepatocellular carcinoma stem cells after down-regulation of Decoy receptor 3.

METHODS: Hepatocellular carcinoma stem cells were obtained from hepatocellular carcinoma cell lines (purchased from the Cell Bank, Chinese Academy of Sciences, Shanghai, China), cultured and passaged. The cells were cultured in the low-glucose DMEM containing nothing (control group), 200 (high-dose group), 100 (middle-dose group) or 50 μmol/L (low-dose group) baicalein. After treatment with baicalein, RT-PCR and western blot were used to detect the expression of decoy receptor 3 at mRNA and protein levels. Cell counting kit-8, flow cytometry, and Transwell assay were used to detect the proliferation, cell cycle distribution, apoptosis and migration of hepatocellular carcinoma stem cells.

RESULTS AND CONCLUSION: Compared with the control group, high-dose baicalein could significantly down-regulated the expression of decoy receptor 3 mRNA and protein in hepatocellular carcinoma stem cells. High-dose baicalein was then confirmed to be the best concentration of action. Compared with the control group, in the high-dose baicalein group, the proliferation ability of hepatocellular carcinoma stem cells decreased significantly, the percentage of S-phase and G₂-phase cells increased, while the percentage of G₁-phase cells decreased (P < 0.05). Compared with the control group, in the high-dose baicalein group, the apoptotic rate of hepatocellular carcinoma stem cells increased significantly, while the migration ability decreased markedly. To conclude, high-dose baicalein can inhibit a series of biological behaviors of hepatocellular carcinoma stem cells by down-regulating the expression of Decoy receptor 3, which provides an experimental basis for clinical treatment of hepatocellular carcinoma with Decoy receptor 3 as the target and hepatocellular carcinoma stem cells as the object.

Key words: baicalein, hepatocellular carcinoma stem cells, decoy receptor 3, cell proliferation, cell cycle, cell apoptosis, cell migration, National Natural Science Foundation of China

CLC Number:

Cen Yanhui, Xia Meng, Jia Wei, Luo Weisheng, Lin Jiang, Chen Songlin, Chen Wei, Liu Peng, Li Mingxing, Li Jingyun, Li Manli, Ai Dingding, Jiang Yunxia.

Baicalein inhibits the biological behavior of hepatocellular carcinoma stem cells by downregulation of Decoy receptor 3 expression [J]. Chinese Journal of Tissue Engineering Research, 2020, 24(7): 1023-1029.

Figures/Tables 12

References

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Baicalein inhibits the biological behavior of hepatocellular carcinoma stem cells by downregulation of Decoy receptor 3 expression

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