Abstract:

BACKGROUND: Nano-hydroxyapatite/chitosan composite has good mechanical properties and biocompatibility, which is suitable for repairing bone defects replacing homograft bone. Basic fibroblast growth factor can improve tissue repair.
OBJECTIVE: To observe the effect of basic fibroblast growth factor combined with nano-hydroxyapatite/chitosan on radial bone defects in rabbits.
METHODS: A total of 36 New Zealand adult healthy rabbits were collected to establish rabbit bone defect models, and then divided into three groups according to different implant materials: implantation of nano-hydroxyapatite/chitosan and basic fibroblast growth factor in experimental group, implantation of nano-hydroxyapatite/chitosan in control group, and no implant materials in blank group.
RESULTS AND CONCLUSION: After 12 weeks of intervention, X-ray examination revealed bone fusion in the implanted area in the experimental group, and bone defects disappeared nearly. Hematoxylin-eosin staining showed that mature lamellar bone, mature Haversian system and bone resorption induced by the proliferation of osteoclasts were found in the experimental group. These results were the repair effects of the experimental group superior to the control group. It has been verified that basic fibroblast growth factor combined with nano-hydroxyapatite/chitosan has a better effect on repairing bone defects, superior to simple nano-hydroxyapatite/chitosan composite.