Effect of Cu2+ and Fe3+ on osteoblast growth and differentiation in hydrogel RADA16-NBD

doi:10.3969/j.issn.2095-4344.2016.16.009

Abstract

Abstract:

BACKGROUND: It is a mature technology to culture MC3T3-E1 cells in the self-assembling peptide hydrogel, RADA16-NBD. Moreover, it is confirmed that a variety of metal ions, such as Fe, Cu, Zn, Mn, are involved in normal bone metabolism.

OBJECTIVE: To observe the effect of Cu²⁺and Fe³⁺ on the proliferation and differentiation of MC3T3-E1 cells cultured in the self-assembling peptide hydrogel, RADA16-NBD.

METHODS: Osteoblasts cultured with RADA16-NBD were divided into three groups and respectively cultured in culture medium containing Cu²⁺, Fe³⁺ or serum-free medium (control group), respectively. After 24, 48 and 72 hours, cell proliferation was detected by cell counting kit-8. After 1, 3, 5 days, alkaline phosphatase activity was detected. At 21 days, formation of calcified nodules was observed. Cell migration ability of cells was observed at 24 hours of Transwill chamber culture.

RESULTS AND CONCLUSION: Compared with the control group, the proliferative ability of cells cultured in the Cu²⁺, Fe³⁺ groups was significantly higher (P < 0.05, P < 0.01). At 72 hours of culture, there was no difference in the cell proliferation among the three groups. At 1, 3, 5 days of culture, the alkaline phosphatase activity in the Cu²⁺, Fe³⁺ groups was significantly higher than that in the control group (P < 0.05); while at 3 and 5 days of culture, the alkaline phosphatase activity in the Cu²⁺ group was significantly higher than that in the Fe³⁺ group (P < 0.05). In addition, the number of migrated cells was higher in the Cu²⁺ group than the Fe³⁺ group (P < 0.05). These findings indicate that both Cu²⁺ and Fe³⁺, especially the former one, can promote MC3T3-E1 cell proliferation, differentiation and migration.
中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Hydrogel, Osteoblasts, Cell Proliferation, Tissue Engineering

Shi Jin-ming, Zhao Gang, Ruan Qiang. Effect of Cu²⁺ and Fe³⁺ on osteoblast growth and differentiation in hydrogel RADA16-NBD[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(16): 2347-2353.

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References

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Effect of Cu²⁺ and Fe³⁺ on osteoblast growth and differentiation in hydrogel RADA16-NBD

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