Isoflurane effects on the proliferation and differentiation of neural stem cells in the hippocampus of neonatal rats

doi:10.3969/j.issn.2095-4344.2016.01.021

Abstract

Abstract:

BACKGROUND: Isoflurane is an anesthesia drug that has a certain effect on the nervous system. It possibly causes neurologic disorders through impacting nerve stem cell function or morphology.
OBJECTIVE: To investigate the effects of isoflurane on the proliferation and differentiation of neural stem cells in the hippocampus of rats.
METHODS: Neural stem cells from the hippocampus of neonatal Sprague-Dawley rats, aged 7 days, were induced and differentiated. Passage 3 cells were obtained and divided into two groups: isoflurane group (a mixture gas of 2.8% isoflurane, 5% CO2 and 95% O2) and control group (a mixture of 5% CO2 and 95% O2). After intervention of 6 hours followed by 2 hours of routine culture, anti-BrdU monoclonal antibody immunofluorescent staining was used to detect cell proliferation, and western blot assay to detect the expression of β3-tubulin and glial fibrillary acidic protein.
RESULTS AND CONCLUSION: Compared with the control group, the number of BrdU positive cells in the isoflurane group reduced significantly, indicating that isoflurane inhibits the proliferation of neural stem cells. Compared with the control group, the expression of glial fibrillary acidic protein in the isoflurane group up-regulated, but the expression of β3-tubulin had no changes, indicating isoflurane promotes the differentiation of neural stem cells into astrocytes.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

CLC Number:

R394.2

Min Na, Hu Qiang-fu, Li Xiao-pei, Nie Xiao-hong, Yang Li-li. Isoflurane effects on the proliferation and differentiation of neural stem cells in the hippocampus of neonatal rats[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(1): 118-122.

Figures/Tables 2

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