Chinese Journal of Tissue Engineering Research ›› 2016, Vol. 20 ›› Issue (1): 89-94.doi: 10.3969/j.issn.2095-4344.2016.01.016

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Application of basic fibroblast growth factor gene-transfected bone marrow mesenchymal stem cells in denervated muscle atrophy

Yu Ning1, Wang Yan-sheng1, Qi Chang-ping2   

  1. 1Fifth Department of Hand Surgery, 2Second Department of Hand Surgery, Central Hospital of Shenyang Medical University, Shenyang 110024, Liaoning Province, China
  • Received:2015-11-15 Online:2016-01-01 Published:2016-01-01
  • Contact: Wang Yan-sheng, Master, Chief physician, Fifth Department of Hand Surgery, Central Hospital of Shenyang Medical University, Shenyang 110024, Liaoning Province, China
  • About author:Yu Ning, Master, Attending physician, Fifth Department of Hand Surgery, Central Hospital of Shenyang Medical University, Shenyang 110024, Liaoning Province, China
  • Supported by:

    the Scientific Plan Project of Liaoning Province, No. 2012225019

Abstract:

BACKGROUND: How to avoid denervated muscular atrophy is a key factor to improve the therapeutic efficacy on peripheral nerve injuries.
OBJECTIVE: To study the effect of basic fibroblast growth factor (bFGF) gene-transfected bone marrow mesenchymal stem cells against denervated muscle atrophy.
METHODS: bFGF genes were transfected into rat bone marrow mesenchymal stem cells using viral transfection method, and then MTT, immunohistochemical staining, hematoxylin-eosin staining, RT-PCR, western blot, and ELISA methods were used to detect the transfection efficiency and product expression. Thirty-two Sprague-Dawley rats were selected to make animal models of sciatic nerve injury, and subjected to multi-point intramuscular injection of bFGF-transfected bone marrow mesenchymal stem cells (experimental group) or cell culture fluid (control group). At 2, 4, 6, 8 weeks after transfection, the gastrocnemius muscle tissues were harvested to detect action potential, residual wet weight, and cross-sectional area of muscle fibers.
RESULTS AND CONCLUSION: The bFGF gene was successfully transfected into bone marrow mesenchymal stem cells using the viral transfection method. The residual wet weight, cross-sectional area and residual action potential of the gastrocnemius muscle were significantly better in the experimental group than the control group (P < 0.05). These findings indicate that bFGF gene-transfected bone marrow mesenchymal stem cells transplanted into the denervated muscle can retard the development of muscle atrophy. 

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